DNA Purification Cell Lysis and Wash Step Modifications for Low‐Template DNA Sample Processing
| Autor: | Jordan O. Cox, Milady T Delacruz, Tracey Dawson Cruz, Madison E Hytinen, Marilyn T. Miller, Sydney I. Menchhoff |
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| Rok vydání: | 2019 |
| Předmět: |
Forensic Genetics
Lysis Touch DNA Polymerase Chain Reaction 01 natural sciences Article Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Genetics Humans 030216 legal & forensic medicine Gene Chromatography Chemistry Cell Membrane 010401 analytical chemistry Extraction (chemistry) Substrate (chemistry) DNA DNA Fingerprinting DNA extraction 0104 chemical sciences Sample collection Microsatellite Repeats |
| Zdroj: | J Forensic Sci |
| ISSN: | 1556-4029 0022-1198 |
| DOI: | 10.1111/1556-4029.14203 |
| Popis: | As DNA technology becomes increasingly sensitive, forensic laboratories are receiving more low-template DNA samples. These samples, already low in DNA content, become even more challenging to process as the available DNA becomes further reduced during the extraction step. In this study, two extraction modifications were tested to determine if the cause of DNA loss could be identified and mitigated. A double lysis technique was used to test for DNA loss in the sample collection substrate, and lysate eluates were re-extracted to determine DNA loss from inefficient binding to the silica column. Both modifications showed DNA was lost at these steps. However, resulting STR profiles from these samples had fewer peaks and lower peak heights when compared to samples processed with no extraction modifications. Overall, the potential benefits of adding these extraction modifications for low-template DNA sample processing are not enough to justify the risk associated with additional manipulation. |
| Databáze: | OpenAIRE |
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