Neisserial outer membrane vesicles bind the coinhibitory receptor carcinoembryonic antigen-related cellular adhesion molecule 1 and suppress CD4+ T lymphocyte function
| Autor: | Denise Halliwell, Henry K. Wong, Ian C. Boulton, Andrew Gorringe, Scott D. Gray-Owen, Ofer Mandelboim, Karen M. Reddin, Hannah S. W. Lee |
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| Rok vydání: | 2007 |
| Předmět: |
CD4-Positive T-Lymphocytes
Immunology Amino Acid Motifs Biology Neisseria meningitidis Microbiology Bacterial Adhesion Jurkat Cells Cell surface receptor Antigens CD Cell Wall Humans Receptors Immunologic Cell adhesion Cells Cultured Neisseria lactamica Immunosuppression Therapy Host Response and Inflammation Cell adhesion molecule Vesicle T lymphocyte biology.organism_classification Growth Inhibitors Bacterial adhesin Infectious Diseases Tyrosine Parasitology Neisseria Bacterial outer membrane Cell Adhesion Molecules Bacterial Outer Membrane Proteins |
| Zdroj: | Infection and immunity. 75(9) |
| ISSN: | 0019-9567 |
| Popis: | Pathogenic Neisseria bacteria naturally liberate outer membrane “blebs,” which are presumed to contribute to pathology, and the detergent-extracted outer membrane vesicles (OMVs) from Neisseria meningitidis are currently employed as meningococcal vaccines in humans. While the composition of these vesicles reflects the bacteria from which they are derived, the functions of many of their constituent proteins remain unexplored. The neisserial colony opacity-associated Opa proteins function as adhesins, the majority of which mediate bacterial attachment to human carcinoembryonic antigen-related cellular adhesion molecules (CEACAMs). Herein, we demonstrate that the Opa proteins within OMV preparations retain the capacity to bind the immunoreceptor tyrosine-based inhibitory motif-containing coinhibitory receptor CEACAM1. When CD4 + T lymphocytes were exposed to OMVs from Opa-expressing bacteria, their activation and proliferation in response to a variety of stimuli were effectively halted. This potent immunosuppressive effect suggests that localized infection will generate a “zone of inhibition” resulting from the diffusion of membrane blebs into the surrounding tissues. Moreover, it demonstrates that OMV-based vaccines must be developed from strains that lack CEACAM1-binding Opa variants. |
| Databáze: | OpenAIRE |
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