CEST MRI detectable liposomal hydrogels for multiparametric monitoring in the brain at 3T
Autor: | Ed X. Wu, Kannie W. Y. Chan, Jianpan Huang, Joseph H. C. Lai, Anthea To, Xiongqi Han, Peng Xiao, Jiadi Xu |
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Rok vydání: | 2020 |
Předmět: |
Cest mri
Medicine (miscellaneous) Mice SCID 02 engineering and technology CEST MRI Mice 03 medical and health sciences Drug treatment Drug Delivery Systems In vivo medicine Animals Humans Multiparametric Magnetic Resonance Imaging Pharmacology Toxicology and Pharmaceutics (miscellaneous) 030304 developmental biology 0303 health sciences Liposome medicine.diagnostic_test Chemistry glioblastoma Brain Hydrogels Magnetic resonance imaging 021001 nanoscience & nanotechnology medicine.disease Single Molecule Imaging Disease Models Animal Liposomes liposome Drug delivery Self-healing hydrogels Female hydrogel 0210 nano-technology Research Paper Biomedical engineering Glioblastoma |
Zdroj: | Theranostics |
ISSN: | 1838-7640 |
Popis: | Adjuvant treatment using local drug delivery is applied in treating glioblastoma multiforme (GBM) after tumor resection. However, there are no non-invasive imaging techniques available for tracking the compositional changes of hydrogel-based drug treatment. Methods: We developed Chemical Exchange Saturation Transfer Magnetic Resonance Imaging (CEST MRI) detectable and injectable liposomal hydrogel to monitor these events in vivo at 3T clinical field. Mechanical attributes of these hydrogels and their in vitro and in vivo CEST imaging properties were systematically studied. Results: The MRI detectable hydrogels were capable of generating multiparametric readouts for monitoring specific components of the hydrogel matrix simultaneously and independently. Herein, we report, for the first time, CEST contrast at -3.4 ppm provides an estimated number of liposomes and CEST contrast at 5 ppm provides an estimated amount of encapsulated drug. CEST contrast decreased by 1.57% at 5 ppm, while the contrast at -3.4 ppm remained constant over 3 d in vivo, demonstrating different release kinetics of these components from the hydrogel matrix. Furthermore, histology analysis confirmed that the CEST contrast at -3.4 ppm was associated with liposome concentrations. Conclusion: This multiparametric CEST imaging of individual compositional changes in liposomal hydrogels, formulated with clinical-grade materials at 3T and described in this study, has the potential to facilitate the refinement of adjuvant treatment for GBM. |
Databáze: | OpenAIRE |
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