Detection of ribose-methylated nucleotides in Pyrodictium occultum tRNA by liquid chromatography—frit-fast atom bombardment mass spectrometry
| Autor: | Naohito Takeda, Akira Tatematsu, Hideo Yoshizumi, Masashi Nakamura |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Nuclease Chromatography biology RNase P Hydrolysis Saccharomyces cerevisiae General Chemistry Ribonucleotides Spectrometry Mass Fast Atom Bombardment Fast atom bombardment Mass spectrometry Archaea Methylation RNA Transfer Phe chemistry.chemical_compound RNA Transfer chemistry Ribose Transfer RNA Mass spectrum biology.protein Nucleotide Chromatography Liquid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 660:223-233 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(94)00299-1 |
| Popis: | Ribose-methylated dinucleotides of the type NmpN′ derived from digestion of tRNA with RNase T 2 were separated and characterized by directly combined liquid chromatography—mass spectrometry (LC—MS) with a continuous-flow frit-fast atom bombardment (frit-FAB) interface. Prediction of NmpN′ peaks was readily made by comparison of the LC profile with that of comparative nuclease P 1 digest. The identity of the candidate peaks including NmpN′ was further recognized by the mass spectra, in which NmpN′ showed intense molecular-related ions, in addition to sequence-specific fragment ions, to verify the chemical structures in both positive- and negative-ion modes. The method was applied to screening NmpN′ (and NmpN′mpN″) in tRNA from the extremely thermophilic archaeon Pyrodictium occultum . |
| Databáze: | OpenAIRE |
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