Human monocyte activation by supernatants from concanavalin A (con A) stimulated lymphocytes
| Autor: | Arnold D. Rubin, Steven D. Douglas, Manfred E. Schmidt |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Lymphocyte
Immunology Centrifugation Cell Fractionation Tritium Monocytes symbols.namesake Phagocytosis medicine Concanavalin A Humans Lymphocytes Serum Albumin Radio-Iodinated Uridine Cells Cultured Cell Nucleus biology Monocyte Metabolism Golgi apparatus Human serum albumin Molecular biology In vitro Microscopy Electron medicine.anatomical_structure Biochemistry Mitogen-activated protein kinase biology.protein symbols Chromatography Gel RNA medicine.drug |
| Zdroj: | Cellular immunology. 9(1) |
| ISSN: | 0008-8749 |
| Popis: | Human peripheral lymphocytes were stimulated with Concanavalin A (Con A) in the absence of serum. Supernatants were collected from control and mitogen stimulated lymphocyte cultures and fractions pooled according to the elution before, together with or after human serum albumin which was added as a marker. Only one fraction derived from Con A stimulated lymphocyte culture Supernatants which eluted immediately after human serum albumin had a significant effect on the metabolism and structure of human monocytes in vitro . Monocytes separated by human serum albumin and incubated with this fraction for 20 hr had an increase in nuclear RNA synthesis. Monocytes attached to cover slips in Leighton tubes showed an increase in the percentage of phagocytizing cells and phagocytic activity. Electron microscopy demonstrated highly phagocytic cells containing numerous Golgi associated granules and strands of nondilated rough surfaced endoplasmic reticulum in presence of the active fraction. |
| Databáze: | OpenAIRE |
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