Cathepsin S in tumours, regional lymph nodes and sera of patients with lung cancer: relation to prognosis
| Autor: | W. Fiehn, Klaus Kayser, B Werle, G Kopitar, A Stremmer, Nina Cimerman, Andreja Sekirnik, Janko Kos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Lung Neoplasms Enzyme-Linked Immunosorbent Assay Biology Cytosol Antibody Specificity medicine Humans Lung cancer MHC-II Lymph node Cathepsin S Cathepsin Respiratory disease Regular Article lung tumours respiratory system medicine.disease Prognosis Cathepsins Immunohistochemistry respiratory tract diseases medicine.anatomical_structure Oncology Lung disease monoclonal antibody ELISA Lymph Lymph Nodes |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Cysteine proteinase cathepsin S (Cat S) is expressed mainly in lymphatic tissues and has been characterised as a key enzyme in major histocompatibility complex class II (MHC-II) mediated antigen presentation. Cat S has been measured in tissue cytosols of lung parenchyma, lung tumours and lymph nodes and in sera of patients with lung tumours and of healthy controls, by specific enzyme-linked immunosorbent assay (ELISA). A difference in Cat S level was found between tumour and adjacent control tissue cytosols of 60 lung cancer patients (median 4.3 vs. 2.8 ng mg−1protein). In lymph nodes obtained from 24 patients of the same group, the level of Cat S was significantly higher than in tumours or lung parenchyma (P< 0.001). Additionally, significantly higher levels were found in non-infiltrated than in infiltrated lymph nodes (median 16.6 vs 7.5 ng mg−1protein). Patients with low levels of Cat S in tumours and lung parenchyma exhibited a significantly higher risk of death than those with high levels of Cat S (P= 0.025 – tumours;P= 0.02 – parenchyma). Immunohistochemical analysis (IHA) of lung parenchyma revealed a staining reaction in alveolar type II cells, macrophages and bronchial epithelial cells. In regional lymph node tissue, strong staining of Cat S was found in lymphocytes and histiocytes. Nevertheless, Cat S was detected also in tumour cells, independently of their origin. Our results provide evidence that Cat S may be involved in malignant progression. Its role, however, differs from that of the related Cats B and L and could be associated with the immune response rather than with remodelling of extracellular matrix. © 2001 Cancer Research Campaign http://www.bjcancer.com |
| Databáze: | OpenAIRE |
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