In utero through lactational exposure to ethinyl estradiol induces cleft phallus and delayed ovarian dysfunction in the offspring
| Autor: | Takako Muroi, Satoko Sakamoto, Kanji Yamasaki, Hideo Mitoma, Shuji Noda, Saori Takakura, Masakuni Sawaki |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Offspring medicine.drug_class Biology Toxicology Ethinyl Estradiol Anovulation Rats Sprague-Dawley Pregnancy Ethinylestradiol Internal medicine medicine Animals Lactation Dose-Response Relationship Drug Ovary Abnormalities Drug-Induced Estrogens Genitalia Female medicine.disease Teratology Rats Endocrinology Estrogen In utero Maternal Exposure Prenatal Exposure Delayed Effects Gestation Female Infertility Female medicine.drug |
| Zdroj: | Toxicological sciences : an official journal of the Society of Toxicology. 75(2) |
| ISSN: | 1096-6080 |
| Popis: | Most of the attention currently focused on endocrine-active chemicals is directed to their effects on the development of offspring exposed to them in utero or during the neonatal period. Pregnant Crj:CD(SD)IGS rats were given ethinyl estradiol (EE) orally in doses of 0.5-50 microg/kg/day from gestational day 7 to postnatal day 18, and their offspring were examined for its effects. Our previous study according to a similar protocol demonstrated the occurrence of cleft phallus in the female offspring exposed to 50 microg/kg of EE in utero and during the lactation period. The present study was designed to assess (1) the reproducibility of the induction of cleft phallus, (2) the fertility of female rats with cleft phallus, and (3) whether any delayed effects, possibly delayed anovulation, were induced. At 50 microg/kg cleft phallus was observed in almost all of the female offspring, and slight retardation of body weight gain was detected in both sexes. At 15-17 weeks of age the animals with cleft phallus could copulate and had fertility comparable to the control group. At 6 months of age, on the other hand, 6/8 of the female offspring at 50 microg/kg exhibited abnormal cyclicity, including persistent estrus, and histological examination revealed follicular cysts and absence of corpora lutea in the ovaries of the rats with persistent estrus. These findings are consistent with delayed anovulation syndrome. The results suggest that observation of cyclicity at 6 months old is able to detect possible delayed ovarian dysfunction induced by perinatal exposure to chemicals. |
| Databáze: | OpenAIRE |
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