Short- and medium-chain carnitine acyltransferases and acyl-CoA thioesterases in mouse provide complementary systems for transport of β-oxidation products out of peroxisomes
| Autor: | Maria A.K. Westin, Stefan E.H. Alexson, Mary C. Hunt |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Molecular Sequence Data Sequence alignment Biology Gene Expression Regulation Enzymologic Mice Cellular and Molecular Neuroscience Acyl-CoA chemistry.chemical_compound Carnitine Acetyltransferase Peroxisomes Animals Amino Acid Sequence Molecular Biology Peptide sequence Pharmacology Regulation of gene expression Base Sequence Carnitine Acyltransferases Biological Transport Cell Biology Peroxisome Catalase Mitochondria Isoenzymes Alternative Splicing chemistry Biochemistry Organ Specificity Molecular Medicine CARNITINE OCTANOYLTRANSFERASE Thiolester Hydrolases Oxidation-Reduction Sequence Alignment |
| Zdroj: | Cellular and Molecular Life Sciences. 65:982-990 |
| ISSN: | 1420-9071 1420-682X |
| Popis: | Peroxisomes metabolize a variety of lipids, acting as a chain-shortening system that produces acyl-CoAs of varying chain lengths, including acetyl-CoA and propionyl-CoA. It is, however, still largely unknown how beta-oxidation products exit peroxisomes and where they are further metabolized. Peroxisomes contain carnitine acetyltransferase (CRAT) and carnitine octanoyltransferase (CROT) that produce carnitine esters for transport out of peroxisomes, together with recently characterized acyl-CoA thioesterases (ACOTs) that produce free fatty acids. Here we have performed tissue expression profiling of the short- and medium-chain carnitine acyltransferases Crat, Crot and the short- and medium-chain thioesterases (Acot12) and (Acot5), and show that they are largely expressed in different tissues, suggesting that they do not compete for the same substrates but rather provide complementary systems for transport of metabolites across the peroxisomal membrane. These data also explain earlier observed tissue differences in peroxisomal production of acetyl-CoA/acetyl-carnitine/acetate and underscores the differences in peroxisome function in various organs. |
| Databáze: | OpenAIRE |
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