Boron neutron capture synovectomy (BNCS) as a potential therapy for rheumatoid arthritis: radiobiological studies at RA-1 Nuclear Reactor in a model of antigen-induced arthritis in rabbits

Autor: Hugo Scolari, Verónica A. Trivillin, David A. Gatti, Sara Feldman, Luis Wentzeis, Amanda E. Schwint, Jorge Castillo, Emiliano C. C. Pozzi, Marcela A. Garabalino, Leandro J. Bruno, Mariela Stur, Andrea Monti Hughes
Rok vydání: 2016
Předmět:
musculoskeletal diseases
Biodistribution
Pathology
medicine.medical_specialty
Necrosis
Ra-1 Nuclear Reactor
CIENCIAS MÉDICAS Y DE LA SALUD
Ovalbumin
medicine.medical_treatment
Biophysics
Arthritis
Ciencias de la Salud
Inflammation
Synovectomy
Rheumatoid Arthritis
Boron Neutron Capture Therapy
Boron Neutron Capture Synovectomy Bncs
030218 nuclear medicine & medical imaging
Arthritis
Rheumatoid
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Decahydrodecaborate (Gb-10)
General Environmental Science
Radiation
medicine.diagnostic_test
Antigen-Induced Arthritis (Aia)
business.industry
Synovial Membrane
Radiobiology
Magnetic resonance imaging
Radiotherapy Dosage
medicine.disease
Otras Ciencias de la Salud
Disease Models
Animal
Effusion
030220 oncology & carcinogenesis
Rheumatoid arthritis
Female
Rabbits
medicine.symptom
Boronophenylalanine (Bpa)
Safety
Nuclear medicine
business
Zdroj: Radiation and environmental biophysics. 55(4)
ISSN: 1432-2099
Popis: Rheumatoid arthritis is a chronic autoimmune pathology characterized by the proliferation and inflammation of the synovium. Boron neutron capture synovectomy (BNCS), a binary treatment modality that combines the preferential incorporation of boron carriers to target tissue and neutron irradiation, was proposed to treat the pathological synovium in arthritis. In a previous biodistribution study, we showed the incorporation of therapeutically useful boron concentrations to the pathological synovium in a model of antigen-induced arthritis (AIA) in rabbits, employing two boron compounds approved for their use in humans, i.e., decahydrodecaborate (GB-10) and boronophenylalanine (BPA). The aim of the present study was to perform low-dose BNCS studies at the RA-1 Nuclear Reactor in the same model. Neutron irradiation was performed post intra-articular administration of BPA or GB-10 to deliver 2.4 or 3.9 Gy, respectively, to synovium (BNCS-AIA). AIA and healthy animals (no AIA) were used as controls. The animals were followed clinically for 2 months. At that time, biochemical, magnetic resonance imaging (MRI) and histological studies were performed. BNCS-AIA animals did not show any toxic effects, swelling or pain on palpation. In BNCS-AIA, the post-treatment levels of TNF-α decreased in four of six rabbits and IFN-γ levels decreased in five of six rabbits. In all cases, MRI images of the knee joint in BNCS-AIA resembled those of no AIA, with no necrosis or periarticular effusion. Synovial membranes of BNCS-AIA were histologically similar to no AIA. BPA-BNCS and GB-10-BNCS, even at low doses, would be therapeutically useful for the local treatment of rheumatoid arthritis. Fil: Trivillin, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina Fil: Bruno, Leandro J.. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Gatti, Claudio David. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Stur, Mariela. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina Fil: Garabalino, Marcela Alejandra. Comisión Nacional de Energía Atómica; Argentina Fil: Monti Hughes, Andrea. Comisión Nacional de Energía Atómica; Argentina Fil: Castillo, Jorge Alberto. Comisión Nacional de Energía Atómica; Argentina Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina Fil: Wentzeis, Luis. Comisión Nacional de Energía Atómica; Argentina Fil: Scolari, Hugo. Comisión Nacional de Energía Atómica; Argentina Fil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina Fil: Feldman, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Databáze: OpenAIRE
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