Quantitative magnetic particle imaging monitors the transplantation, biodistribution, and clearance of stem cells in vivo

Autor: Elaine W. Yu, Beliz Gunel, Tandis Vazin, Patrick W. Goodwill, Bo Zheng, Marc Philip von See, Kuan Lu, Steven M. Conolly, David V. Schaffer
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Diagnostic Imaging
Pathology
medicine.medical_specialty
Biodistribution
Medicine (miscellaneous)
Spleen
02 engineering and technology
Mesenchymal Stem Cell Transplantation
Ferric Compounds
030218 nuclear medicine & medical imaging
03 medical and health sciences
Magnetics
Mice
0302 clinical medicine
Magnetic particle imaging
In vivo
medicine
Animals
Humans
Tissue Distribution
ddc:610
Pharmacology
Toxicology and Pharmaceutics (miscellaneous)
Technik [600]
mesenchymal stem cells
Staining and Labeling
cell therapy tracking
business.industry
Mesenchymal stem cell
021001 nanoscience & nanotechnology
Rats
Inbred F344
3. Good health
Transplantation
medicine.anatomical_structure
Medizin [610]
quantitative imaging
magnetic particle imaging
Nanoparticles
Administration
Intravenous
Female
Stem cell
0210 nano-technology
business
ddc:600
Ex vivo
Research Paper
Zdroj: Theranostics 3 (6): 291-301 (2016-01-01)
Theranostics
Popis: Stem cell therapies have enormous potential for treating many debilitating diseases, including heart failure, stroke and traumatic brain injury. For maximal efficacy, these therapies require targeted cell delivery to specific tissues followed by successful cell engraftment. However, targeted delivery remains an open challenge. As one example, it is common for intravenous deliveries of mesenchymal stem cells (MSCs) to become entrapped in lung microvasculature instead of the target tissue. Hence, a robust, quantitative imaging method would be essential for developing efficacious cell therapies. Here we show that Magnetic Particle Imaging (MPI), a novel technique that directly images iron-oxide nanoparticle-tagged cells, can longitudinally monitor and quantify MSC administration in vivo. MPI offers near-ideal image contrast, depth penetration, and robustness; these properties make MPI both ultra-sensitive and linearly quantitative. Here, we imaged, for the first time, the dynamic trafficking of intravenous MSC administrations using MPI. Our results indicate that labeled MSC injections are immediately entrapped in lung tissue and then clear to the liver within one day, whereas standard iron oxide particle (Resovist) injections are immediately taken up by liver and spleen. Longitudinal MPI-CT imaging also indicated a clearance half-life of MSC iron oxide labels in the liver at 4.6 days. Finally, our ex vivo MPI biodistribution measurements of iron in liver, spleen, heart, and lungs after injection showed excellent agreement (R(2) = 0.943) with measurements from induction coupled plasma spectrometry. These results demonstrate that MPI offers strong utility for noninvasively imaging and quantifying the systemic distribution of cell therapies and other therapeutic agents.
Databáze: OpenAIRE
Externí odkaz: