The MicroRNA miR-1 Regulates a MEF-2-Dependent Retrograde Signal at Neuromuscular Junctions
| Autor: | John Kim, Annie L. Conery, David J. Simon, Katherine L. Thompson-Peer, Gary Ruvkun, Joshua M. Kaplan, Michael J. Soskis, Jon M. Madison |
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| Rok vydání: | 2008 |
| Předmět: |
Transcription
Genetic rab3 GTP-Binding Proteins Neuromuscular Junction Receptors Nicotinic Biology Synaptic vesicle MOLNEURO Article General Biochemistry Genetics and Molecular Biology Neuromuscular junction 03 medical and health sciences 0302 clinical medicine Postsynaptic potential medicine Animals Nicotinic Agonists Caenorhabditis elegans Caenorhabditis elegans Proteins Transcription factor 030304 developmental biology 0303 health sciences Biochemistry Genetics and Molecular Biology(all) Cell biology MicroRNAs Nicotinic acetylcholine receptor Nicotinic agonist medicine.anatomical_structure Levamisole SIGNALING Mutation RNA Signal transduction 030217 neurology & neurosurgery Acetylcholine Signal Transduction Transcription Factors medicine.drug |
| Zdroj: | Cell. 133:903-915 |
| ISSN: | 0092-8674 |
| Popis: | SummaryWe show that miR-1, a conserved muscle-specific microRNA, regulates aspects of both pre- and postsynaptic function at C. elegans neuromuscular junctions. miR-1 regulates the expression level of two nicotinic acetylcholine receptor (nAChR) subunits (UNC-29 and UNC-63), thereby altering muscle sensitivity to acetylcholine (ACh). miR-1 also regulates the muscle transcription factor MEF-2, which results in altered presynaptic ACh secretion, suggesting that MEF-2 activity in muscles controls a retrograde signal. The effect of the MEF-2-dependent retrograde signal on secretion is mediated by the synaptic vesicle protein RAB-3. Finally, acute activation of levamisole-sensitive nAChRs stimulates MEF-2-dependent transcriptional responses and induces the MEF-2-dependent retrograde signal. We propose that miR-1 refines synaptic function by coupling changes in muscle activity to changes in presynaptic function. |
| Databáze: | OpenAIRE |
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