Nonlinear ultrasound imaging of nanoscale acoustic biomolecules
| Autor: | Mikhail G. Shapiro, Johan M. Melis, Anupama Lakshmanan, Audrey Lee-Gosselin, David Maresca, Dennis M. Kochmann, Yu-Li Ni, Raymond W. Bourdeau |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Nanostructure Physics and Astronomy (miscellaneous) business.industry Ultrasound Pulse sequence Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology Amplitude modulation 03 medical and health sciences Nonlinear system 030104 developmental biology Biophysics and Bio-Inspired Systems Nanomedicine Contrast ratio 0210 nano-technology Sound pressure business Biomedical engineering |
| Zdroj: | Applied Physics Letters. 110:073704 |
| ISSN: | 1077-3118 0003-6951 |
| DOI: | 10.1063/1.4976105 |
| Popis: | Ultrasound imaging is widely used to probe the mechanical structure of tissues and visualize blood flow. However, the ability of ultrasound to observe specific molecular and cellular signals is limited. Recently, a unique class of gas-filled protein nanostructures called gas vesicles (GVs) was introduced as nanoscale (∼250 nm) contrast agents for ultrasound, accompanied by the possibilities of genetic engineering, imaging of targets outside the vasculature and monitoring of cellular signals such as gene expression. These possibilities would be aided by methods to discriminate GV-generated ultrasound signals from anatomical background. Here, we show that the nonlinear response of engineered GVs to acoustic pressure enables selective imaging of these nanostructures using a tailored amplitude modulation strategy. Finite element modeling predicted a strongly nonlinear mechanical deformation and acoustic response to ultrasound in engineered GVs. This response was confirmed with ultrasound measurements in the range of 10 to 25 MHz. An amplitude modulation pulse sequence based on this nonlinear response allows engineered GVs to be distinguished from linear scatterers and other GV types with a contrast ratio greater than 11.5 dB. We demonstrate the effectiveness of this nonlinear imaging strategy in vitro, in cellulo, and in vivo. |
| Databáze: | OpenAIRE |
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