Fiber-type-specific sensitivities and phenotypic adaptations to dietary fat overload differentially impact fast- versus slow-twitch muscle contractile function in C57BL/6J mice
| Autor: | Jolita Ciapaite, Sjoerd A.A. van den Berg, Ko Willems van Dijk, Sander M. Houten, Klaas Nicolay, Jeroen A. L. Jeneson |
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| Přispěvatelé: | Paediatric Metabolic Diseases, Laboratory Genetic Metabolic Diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Endocrinology Diabetes and Metabolism Muscle Relaxation Clinical Biochemistry SDG 3 – Goede gezondheid en welzijn Biochemistry Acyl-CoA Dehydrogenase chemistry.chemical_compound Random Allocation MITOCHONDRIA Myosin TROPONIN-T Diet Fat-Restricted INSULIN-RESISTANCE OXIDATIVE CAPACITY Nutrition and Dietetics Fatty Acids Skeletal muscle function High-fat-diet-induced obesity food and beverages CHAIN ACYL-COA musculoskeletal system Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Phospholamban medicine.anatomical_structure Muscle Fibers Slow-Twitch Allostasis Muscle Fibers Fast-Twitch SKELETAL-MUSCLE Glycogen Muscle Contraction medicine.medical_specialty Dietary lipid ISOFORMS Biology METABOLISM Diet High-Fat Insulin resistance Troponin T SDG 3 - Good Health and Well-being Internal medicine Carnitine FED RATS medicine Animals Oxidative phosphorylation Obesity Molecular Biology Soleus muscle Fatty acid metabolism Skeletal muscle medicine.disease SARCOPLASMIC-RETICULUM Mitochondria Muscle Mice Inbred C57BL Endocrinology chemistry Electron Transport Chain Complex Proteins Fiber type Stearoyl-CoA desaturase-1 Transcription Factors |
| Zdroj: | Journal of Nutritional Biochemistry, 26(2), 155-164 Journal of Nutritional Biochemistry, 26(2), 155-164. ELSEVIER SCIENCE INC Journal of Nutritional Biochemistry, 26(2), 155-164. Elsevier Journal of nutritional biochemistry, 26(2), 155-164. Elsevier Inc. |
| ISSN: | 0955-2863 |
| Popis: | High-fat diets (HFDs) have been shown to interfere with skeletal muscle energy metabolism and cause peripheral insulin resistance. However, understanding of HFD impact on skeletal muscle primary function, i.e., contractile performance, is limited. Male C57BL/6J mice were fed HFD containing lard (HFL) or palm oil (HFP), or low-fat diet (LED) for 5 weeks. Fast-twitch (FT) extensor digitorum Iongus (EDL) and slow-twitch (ST) soleus muscles were characterized with respect to contractile function and selected biochemical features. In EDL muscle, a 30%-50% increase in fatty acid (FA) content and doubling of long-chain acylcarnitine (C14-C18) content in response to HFL and HFP feeding were accompanied by increase in protein levels of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha, mitochondrial oxidative phosphorylation complexes and acyl-CoA dehydrogenases involved in mitochondrial FA beta-oxidation. Peak force of FT EDL twitch and tetanic contractions was unaltered, but the relaxation time (RT) of twitch contractions was 30% slower compared to LFD controls. The latter was caused by accumulation of lipid intermediates rather than changes in the expression levels of proteins involved in calcium handling. In ST soleus muscle, no evidence for lipid overload was found in any HFD group. However, particularly in HFP group, the peak force of twitch and tetanic contractions was reduced, but RT was faster than LFD controls. The latter was associated with a fast-to-slow shift in troponin T isoform expression. Taken together, these data highlight fiber-type-specific sensitivities and phenotypic adaptations to dietary lipid overload that differentially impact fast- versus slow-twitch skeletal muscle contractile function. (C) 2015 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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