Effects of estradiol and progesterone on the proinflammatory cytokine production by mononuclear cells from patients with chronic hepatitis C
| Autor: | Mi Shen, Masayuki Shono, Ichiro Shimizu, Hidetaka Takenaka, Katsutaka Sannomiya, Mari Urata, Nao Kohno, Eriko Aoyagi, Tatuzo Itagaki, Ying Yuan, Tetsuji Takayama, Katsuyoshi Tamaki |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Inflammation Models Biological Peripheral blood mononuclear cell Proinflammatory cytokine Internal medicine medicine Humans Macrophage Progesterone Aged Estradiol business.industry Gastroenterology Interleukin General Medicine Hepatitis C Hepatitis C Chronic Middle Aged medicine.disease Postmenopause Cytokine Endocrinology Premenopause Case-Control Studies Leukocytes Mononuclear Cytokines Female Tumor necrosis factor alpha medicine.symptom business Rapid Communication |
| Zdroj: | World Journal of Gastroenterology. 14:2200 |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.14.2200 |
| Popis: | AIM: To investigate the effects of estradiol (E2) and progesterone on the unstimulated and oxidative stress-stimulated production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-8, and macrophage chemotactic protein (MCP)-1 by peripheral blood mononuclear cells (PBMCs) from patients with chronic hepatitis C and healthy controls. METHODS: The PBMCs were separated from age-matched 72 males and 71 females with and without chronic hepatitis C, who were divided into two groups based on a mean menopausal age of 50 years. Oxidative stress was induced by hydrogen peroxide in the cells incubated in serum-free media. Cytokines in the culture supernatant were measured by an enzyme-linked immunosorbent assay. RESULTS: The highest levels of the spontaneous production of TNF-α, IL-1β, IL-8, and MCP-1 by the unstimulated PBMCs were in the older male patients with chronic hepatitis C and the lowest levels were in the pre-menopausal female healthy controls. E2 inhibited the cytokine production by the unstimulated PBMCs from the older male and post-menopausal female patients, which was further stimulated by progesterone. The exposure to hydrogen peroxide in the PBMCs from the younger male and pre-menopausal female healthy subjects induced the production of cytokines. The change rates of the hydrogen peroxide-stimulated cytokine production were suppressed by E2 and enhanced by progesterone. CONCLUSION: These findings suggest that E2 may play a favorable role in the course of persistent liver injury by preventing the accumulation of monocytes-macrophages and by inhibiting proinflammatory cytokine production, whereas progesterone may counteract the favorable E2 effects. |
| Databáze: | OpenAIRE |
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