| Autor: |
Asmaa Lachhab, Corinne Barat, Stéphane Bolduc, Ingrid Saba, Hazem Orabi, Michel J. Tremblay, Weronika Jakubowska, Stéphane Chabaud, Martin Pelletier, Maude Leclerc, Nolan Reyjon |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Tissue engineering. Part C, Methods. 27(3) |
| ISSN: |
1937-3392 |
| Popis: |
The lack of appropriate experimental models often limits our ability to investigate the establishment of infections in specific tissues. To reproduce the structural and spatial organization of vaginal mucosae to study human immunodeficiency virus type-1 (HIV-1) infection, we used the self-assembly technique to bioengineer tridimensional vaginal mucosae using human cells extracted from HIV-1-negative healthy pre- and postmenopausal donors. We produced a stroma, free of exogenous material, that can be adapted to generate near-to-native vaginal tissue with the best complexity obtained with seeded epithelial cells on the organ-specific stroma. The autologous engineered tissues had mechanical properties close to native mucosa and shared similar glycogen production, which declined in reconstructed tissues of the postmenopausal donor. The |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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