Histamine stimulates secretion of extracellular vesicles with nucleotidase activity in rat submandibular gland
| Autor: | Emmanuel Quinteros Villarruel, Betina Orman, Graciela Mabel Stranieri, María Belén Benítez, Débora A. González, Macarena Brandt, Martín M. Barbieri van Haaster |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Indoles Nucleotidase activity Submandibular Gland Biology In Vitro Techniques Piperazines 03 medical and health sciences chemistry.chemical_compound Histamine receptor Extracellular Vesicles Microscopy Electron Transmission ATP hydrolysis Nucleotidases Nucleotidase medicine Extracellular Animals Rats Wistar General Dentistry 030102 biochemistry & molecular biology Cell Biology General Medicine Purinergic signalling Submandibular gland Rats 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology chemistry Biochemistry Histamine |
| Zdroj: | Archives of oral biology. 85 |
| ISSN: | 1879-1506 |
| Popis: | Background Extracellular vesicles released by different cells have been isolated from diverse fluids including saliva. We previously reported that rat submandibular glands secrete nanovesicles that catalyze hydrolysis of ATP, ADP and AMP, which are actors of the purinergic signaling system along with adenosine. Extracellular nucleotides like ATP and adenosine are involved in the regulation of inflammatory processes and apoptosis. Histamine, a widely distributed biogenic amine, is involved in inflammatory response. Objective To test if activation of histamine receptors in rat submandibular gland promotes changes in the release of vesicles with nucleotidase activity that could modulate purinergic signaling. Methods Rat submandibular glands were incubated in the absence or presence of histamine and JNJ7777120, an antagonist for H4 receptors. Extracellular vesicles were isolated from incubation media by differential centrifugation. Vesicular nucleotidase activity was measured following Pi release by 3 mM MgATP, MgADP or MgAMP. Results Histamine increased the release of vesicles with nucleotidase activity in a concentration dependent manner. JNJ7777120 significantly reduced this effect. Vesicular nucleotidases obtained in the absence or presence of histamine promoted Pi production from ATP, ADP and AMP. Conclusion The results show a relationship between histamine and the regulation of purinergic signaling, which could be important in the modulation of inflammatory processes. |
| Databáze: | OpenAIRE |
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