Mycobacterium haemophilum and lymphadenitis in children
| Autor: | Ed J. Kuijper, Jerome A. Lindeboom, Jan M. Prins, Lesla E. S. Bruijnesteijn van Coppenraet, Eric C. J. Claas |
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| Přispěvatelé: | Other Research, Oral and Maxillofacial Surgery, Amsterdam institute for Infection and Immunity, Infectious diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Epidemiology Biopsy lcsh:Medicine Polymerase Chain Reaction Sensitivity and Specificity law.invention lcsh:Infectious and parasitic diseases Species Specificity law lymphadenitis medicine Humans lcsh:RC109-216 MGB Child minor groove binder Pathogen Polymerase chain reaction Mycobacterium Infections research biology medicine.diagnostic_test Biopsy Needle lcsh:R biology.organism_classification Virology Mycobacterium haemophilum Infectious Diseases Real-time polymerase chain reaction Amplicon sequencing real-time PCR Mycobacterium |
| Zdroj: | Emerging Infectious Diseases, Vol 11, Iss 1, Pp 62-68 (2005) Emerging infectious diseases, 11(1), 62-68. Centers for Disease Control and Prevention (CDC) Emerging Infectious Diseases |
| ISSN: | 1080-6040 |
| Popis: | Mycobacterium haemophilum is the second most common pathogen in children with mycobacterial lymphadenitis. Infections associated with Mycobacterium haemophilum are underdiagnosed because specific culture methods required for its recovery are not applied routinely. Using polymerase chain reaction (PCR) technology on fine needle aspirates and biopsied specimens from 89 children with cervicofacial lymphadenitis, we assessed the importance of M. haemophilum. Application of a Mycobacterium genus–specific real-time PCR in combination with amplicon sequencing and a M. haemophilum–specific PCR resulted in the recognition of M. haemophilum as the causative agent in 16 (18%) children with cervicofacial lymphadenitis. Mycobacterium avium was the most frequently found species (56%), and M. haemophilum was the second most commonly recognized pathogen. Real-time PCR results were superior to culture because only 9 (56%) of the 16 diagnosed M. haemophilum infections were positive by culture. |
| Databáze: | OpenAIRE |
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