Mechanistic background and clinical applications of indocyanine green fluorescence imaging of hepatocellular carcinoma
| Autor: | Kiyoshi Hasegawa, Shingo Tsuji, Yutaka Midorikawa, Junichi Kaneko, Shouichi Satou, Masashi Fukayama, Yasuteru Urano, Yoshikuni Kawaguchi, Norihiro Kokudo, Koichi Masuda, Takeaki Ishizawa, Junji Shibahara, Hiroyuki Aburatani |
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| Rok vydání: | 2013 |
| Předmět: |
Diagnostic Imaging
Indocyanine Green Fluorescence-lifetime imaging microscopy Pathology medicine.medical_specialty Carcinoma Hepatocellular genetic structures Contrast Media Organic Anion Transporters Sodium-Dependent Organic Anion Transporters Sodium-Independent Fluorescence Immunoenzyme Techniques chemistry.chemical_compound Solute Carrier Organic Anion Transporter Family Member 1B3 Monitoring Intraoperative Preoperative Care medicine Carcinoma Fluorescence microscope Biomarkers Tumor Humans Symporters business.industry Liver Neoplasms HCCS medicine.disease Prognosis eye diseases digestive system diseases Oncology chemistry Microscopy Fluorescence Hepatocellular carcinoma Hepatocytes Immunohistochemistry Surgery Liver cancer business Indocyanine green |
| Zdroj: | Annals of surgical oncology. 21(2) |
| ISSN: | 1534-4681 |
| Popis: | Although clinical applications of intraoperative fluorescence imaging of liver cancer using indocyanine green (ICG) have begun, the mechanistic background of ICG accumulation in the cancerous tissues remains unclear. In 170 patients with hepatocellular carcinoma cells (HCC), the liver surfaces and resected specimens were intraoperatively examined by using a near-infrared fluorescence imaging system after preoperative administration of ICG (0.5 mg/kg i.v.). Microscopic examinations, gene expression profile analysis, and immunohistochemical staining were performed for HCCs, which showed ICG fluorescence in the cancerous tissues (cancerous-type fluorescence), and HCCs showed fluorescence only in the surrounding non-cancerous liver parenchyma (rim-type fluorescence). ICG fluorescence imaging enabled identification of 273 of 276 (99 %) HCCs in the resected specimens. HCCs showed that cancerous-type fluorescence was associated with higher cancer cell differentiation as compared with rim-type HCCs (P |
| Databáze: | OpenAIRE |
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