A mouse model reproducing the pathophysiology of neonatal group B streptococcal infection
| Autor: | Paula Ferreira, Camila C. Portugal, Arnaud Firon, Ana Puga, Patrick Trieu-Cuot, Joana Bravo, Adília Ribeiro, Elva Bonifácio Andrade, Madalena Costa, Augusto Faustino, Teresa Summavielle, Margarida Correia-Neves, Ana Magalhães |
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| Přispěvatelé: | Repositório Científico do Instituto Politécnico do Porto, Instituto de Investigação e Inovação em Saúde, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto = University of Porto, Instituto de Investigação e Inovação em Saúde (I3S), Instituto de Biologia Molecular e Celular - institute for molecular and cell biology [Porto, Portugal] (IBMC), Instituto Politécnico do Porto = Polytechnic Institute of Porto, Instituto de Biologia Molecular e Celular (IBMC), Life and Health Sciences Research Institute [Braga] (ICVS), University of Minho [Braga], Karolinska Institutet [Stockholm], Biologie des Bactéries pathogènes à Gram-positif - Biology of Gram-Positive Pathogens, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by funds from Foundation for Science and Technology (FCT), European Regional Development Fund (FEDER) and Compete under project POCI-01-0145-FEDER-016607 (PTDC/IMI-MIC/1049/2014) and from the project NORTE-01-0145-FEDER-000012, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). T.S. and A.M. were supported by Investigador FCT (IF/00875/2012 and IF/00753/2014), POPH and Fundo Social Europeu. E.B.A. and C.C.P. hold postdoctoral fellowships from FCT (PTDC/IMI-MIC/1049/2014 and SFRH/BPD/91962/2012). Ar.F. and P.T.C. were supported by Laboratoire d’Excellence (LABEX) Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Universidade do Porto, Instituto Politécnico do Porto = Oporto Polytechnic Institute, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Instituto de Ciências Biomédicas Abel Salazar, Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
MESH: Inflammation 0301 basic medicine [SDV]Life Sciences [q-bio] General Physics and Astronomy medicine.disease_cause MESH: Animals Newborn Group B Hemolysin Proteins Mice MESH: Pregnancy MESH: Streptococcal Infections Pregnancy MESH: Behavior Animal MESH: Animals lcsh:Science Mice Inbred BALB C Multidisciplinary Behavior Animal Transmission (medicine) MESH: Pregnancy Animal 3. Good health MESH: Infectious Disease Transmission Vertical MESH: Meningitis Bacterial [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Hemolysin Proteins Vagina Female Meningitis Offspring Science MESH: Mice Inbred BALB C Group B Streptococcal Infection Virulence Biology Article General Biochemistry Genetics and Molecular Biology Meningitis Bacterial Streptococcus agalactiae 03 medical and health sciences MESH: Meningitis Streptococcal Infections medicine Animals Maze Learning MESH: Mice Inflammation Science & Technology Perforin MESH: Maze Learning Body Weight General Chemistry medicine.disease MESH: Streptococcus agalactiae [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Male Infectious Disease Transmission Vertical MESH: Perforin MESH: Body Weight Disease Models Animal 030104 developmental biology Animals Newborn MESH: Vagina Immunology Pregnancy Animal lcsh:Q Cytolysin MESH: Disease Models Animal MESH: Female |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Nature Communications Nature Communications, 2018, 9 (1), pp.3138. ⟨10.1038/s41467-018-05492-y⟩ Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018) Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.3138. ⟨10.1038/s41467-018-05492-y⟩ |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-05492-y⟩ |
| Popis: | Group B streptococcal (GBS) meningitis remains a devastating disease. The absence of an animal model reproducing the natural infectious process has limited our understanding of the disease and, consequently, delayed the development of effective treatments. We describe here a mouse model in which bacteria are transmitted to the offspring from vaginally colonised pregnant females, the natural route of infection. We show that GBS strain BM110, belonging to the CC17 clonal complex, is more virulent in this vertical transmission model than the isogenic mutant BM110∆cylE, which is deprived of hemolysin/cytolysin. Pups exposed to the more virulent strain exhibit higher mortality rates and lung inflammation than those exposed to the attenuated strain. Moreover, pups that survive to BM110 infection present neurological developmental disability, revealed by impaired learning performance and memory in adulthood. The use of this new mouse model, that reproduces key steps of GBS infection in newborns, will promote a better understanding of the physiopathology of GBS-induced meningitis. The authors gratefully acknowledge the help of Encarnaca̧ ̃o Ribeiro for excellent technical assistance, Joana Tavares for assisting with IVIS Lumina LT, Susana Roque for the luminex instrument experiments, the Molecular Microbiology group at i3S for microscope use, and the Portuguese architect and artist Gil Ferreira da Silva for the artworks included in the last figure. This work was supported by funds from Foundation for Science and Technology (FCT), European Regional Development Fund (FEDER) and Compete under project POCI-01-0145-FEDER-016607 (PTDC/IMI-MIC/1049/2014) and from the project NORTE-01-0145-FEDER-000012, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). T.S. and A.M. were supported by Investigador FCT (IF/00875/2012 and IF/00753/2014), POPH and Fundo Social Europeu. E.B.A. and C.C.P. hold postdoctoral fellowships from FCT (PTDC/IMI-MIC/1049/2014 and SFRH/BPD/91962/2012). Ar.F. and P.T.C. were supported by Laboratoire d’Excellence (LABEX) Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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