The Clathrin Adaptor Complex AP-2 Mediates Endocytosis of BRASSINOSTEROID INSENSITIVE1 inArabidopsis
| Autor: | Dominique Eeckhout, Isabelle Vanhoutte, Zheng-Yi Xu, Geert De Jaeger, Eugenia Russinova, Jürgen Kleine-Vehn, Sibu Simon, Kyungyoung Song, Jiří Friml, Wim Dejonghe, Soo Youn Kim, Astrid Gadeyne, Geert Persiau, Niloufer G. Irani, Daniël Van Damme, Inhwan Hwang, Simone Di Rubbo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Clathrin adaptor complex education Adaptor Protein Complex 2 Arabidopsis Plant Science Endocytosis Plant Roots 01 natural sciences Clathrin 03 medical and health sciences chemistry.chemical_compound Brassinosteroid Research Articles 030304 developmental biology 0303 health sciences biology Arabidopsis Proteins Cell Membrane fungi Signal transducing adaptor protein Cell Biology Receptor-mediated endocytosis biology.organism_classification Cell biology Protein Transport chemistry biology.protein Clathrin adaptor proteins Protein Kinases Protein Binding Signal Transduction 010606 plant biology & botany |
| Zdroj: | The Plant Cell |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.113.114058 |
| Popis: | Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance of this process, the machinery that regulates CME in plants is largely unknown. In mammals, the heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana, the biochemistry and functionality of the complex is still uncharacterized. Here, we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the large AP-2 subunits, AP2A1, localized at the PM and interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1), was shown to depend on AP-2. Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis is evolutionarily conserved and that AP-2 functions in receptor-mediated endocytosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|