Molecular Routes to Specific Identification of the Lactobacillus Casei Group at the Species, Subspecies and Strain Level

Autor: Elwira Komoń-Janczara, Agnieszka Korzeniowska-Kowal, Monika Kordowska-Wiater, Michał Dworniczak, Piotr Jarocki, Anna Wzorek, Monika Pytka, Agnieszka Glibowska
Rok vydání: 2020
Předmět:
0301 basic medicine
Polymerase Chain Reaction
L. casei group
law.invention
lcsh:Chemistry
L. rhamnosus
Probiotic
law
Amplified Fragment Length Polymorphism Analysis
lcsh:QH301-705.5
Spectroscopy
L. casei
food and beverages
differentiation
General Medicine
Computer Science Applications
Lacticaseibacillus casei
Diarrhea
medicine.symptom
DNA
Bacterial
Lactobacillus casei
Lactobacillus paracasei
030106 microbiology
Biology
Article
Catalysis
Microbiology
Inorganic Chemistry
03 medical and health sciences
Lactobacillus rhamnosus
medicine
Humans
Typing
Physical and Theoretical Chemistry
Molecular Biology
L. paracasei
Lactose intolerance
Organic Chemistry
biology.organism_classification
medicine.disease
Molecular Typing
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
probiotics
identification
Multiplex Polymerase Chain Reaction
Bacteria
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 8
International Journal of Molecular Sciences, Vol 21, Iss 2694, p 2694 (2020)
ISSN: 1422-0067
Popis: The genus Lactobacillus includes, among others, Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus, species that are collectively referred to as the Lactobacillus casei group. Many studies have shown that strains belonging to this group may decrease lactose intolerance, the effects of inflammatory bowel disease, diarrhea, constipation, food allergies and even colon cancer. Moreover, evidences exists of positive effects of these bacteria on mucosal immunity and blood cholesterol level. Because of their beneficial influence on human health, many of them are used as food additives and probiotic pharmaceuticals. It should be stressed that health-promoting properties are not attributed at the species level, but to specific strains. Therefore, procedures are necessary to allow specific identification at each phylogenetic level&mdash
genus, species and strain. In this paper we present a practical overview of molecular methods for the identification and differentiation of L. casei bacteria. The research included 30 bacterial strains belonging to three species: L.casei, L. paracasei and L. rhamnosus. Among the tested procedures were genus- and species-specific PCR, multiplex-PCR, Real-Time HRM analysis, RFLP-PCR, rep-PCR, RAPD-PCR, AFLP-PCR, and proteomic methods such as MALDI-TOF MS typing and SDS-PAGE fingerprinting. The obtained results showed that multiplex-PCR and MALDI-TOF MS turned out to be the most useful methods to identify the tested bacteria at the species level. At the strain level, the AFLP-PCR method showed the highest discriminatory power. We hope that the presented results will allow for the easy selection of an appropriate procedure, depending on the experiment conducted and the equipment capabilities of any given laboratory.
Databáze: OpenAIRE
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