Physicochemical properties of anodized-hydrothermally treated titanium with a nanotopographic surface structure promote osteogenic differentiation in dental pulp stem cells
| Autor: | Masaki Hata, Ryohei Matsukawa, Atsushi Aoyagi, Yuka Imanishi, Jun Takebe |
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| Rok vydání: | 2021 |
| Předmět: |
Bone sialoprotein
0206 medical engineering 02 engineering and technology Matrix (biology) Osseointegration Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine stomatognathic system Osteogenesis Dental pulp stem cells Animals Dentistry (miscellaneous) Nanotopography Osteopontin Bone regeneration Dental Pulp Titanium biology Chemistry Stem Cells Cell Differentiation 030206 dentistry 020601 biomedical engineering Cell biology Rats Osteocalcin biology.protein Oral Surgery |
| Zdroj: | Journal of prosthodontic research. 65(4) |
| ISSN: | 2212-4632 |
| Popis: | Purpose Implants made of anodized-hydrothermally treated commercially pure titanium with a nanotopographic surface structure (SA-treated c.p.Ti) may advantageously promote contact osteogenesis during the early stages of healing. We hypothesized that utilizing SA-treated c.p.Ti with dental pulp stem cells (DPSCs) might improve osteoconduction during the process of osseointegration. This in vitro study investigated the effect of initial adhesion of DPSCs to SA-treated c.p.Ti compared with conventional c.p.Ti and anodic oxide (AO) c.p.Ti.Methods DPSCs were obtained from the mandibular incisors of Sprague-Dawley rats and cultured without osteogenic induction medium on c.p.Ti, AO c.p.Ti, and SA-treated c.p.Ti disks for up to 14 days. The morphology, proliferation, and differentiation of DPSCs were assessed by scanning electron microscopy, an MTT assay, and Alizarin Red S staining, respectively. A real-time quantitative polymerase chain reaction was used to quantify the mRNA expression of osteocalcin, osteopontin, and bone sialoprotein.Results On all disks, the DPSCs appeared flattened with the formation of extensions over time. The filopodium-like extensions were closely bound to the SA-treated c.p.Ti surface. The proliferation of DPSCs was not significantly different among the c.p.Ti treatments. However, DPSCs on SA-treated c.p.Ti showed the greatest mRNA levels of osteopontin, osteocalcin, and bone sialoprotein, as well as increased Alizarin Red S staining.Conclusions The results of the present in vitro study demonstrate that the surface properties of SA-treated c.p.Ti disks enhance osteogenic differentiation of DPSCs and may facilitate mineralized matrix formation on SA-treated c.p.Ti implant surfaces, which can enhance early bone regeneration. |
| Databáze: | OpenAIRE |
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