Lhx1 is required for specification of the renal progenitor cell field

Autor: Caroline E. Haldin, Neil A. Hukriede, Carsten Stuckenholz, Sung-Kook Hong, Chiara Cianciolo Cosentino, Igor B. Dawid, Zhao Hui, Xiongfong Chen, M. Cecilia Cirio
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Embryology
Embryo
Nonmammalian
Time Factors
Xenopus
Organogenesis
lcsh:Medicine
Xenopus Proteins
Kidney
Mesoderm
0302 clinical medicine
Morphogenesis
Pattern Formation
lcsh:Science
Oligonucleotide Array Sequence Analysis
0303 health sciences
Multidisciplinary
Stem Cells
Gene Expression Regulation
Developmental
Embryo
Cell Differentiation
Animal Models
medicine.anatomical_structure
embryonic structures
Intermediate mesoderm
Research Article
animal structures
Mesenchyme
LIM-Homeodomain Proteins
Biology
03 medical and health sciences
Organ Culture Techniques
Model Organisms
Xenopus Laevis
medicine
Animals
Gene Silencing
030304 developmental biology
Body Patterning
Cell Proliferation
Homeodomain Proteins
urogenital system
lcsh:R
Oligonucleotides
Antisense
Molecular Development
Molecular biology
Signaling
Homeobox
lcsh:Q
Organism Development
030217 neurology & neurosurgery
Transcription Factors
Developmental Biology
Zdroj: PLoS ONE, Vol 6, Iss 4, p e18858 (2011)
PLoS ONE
ISSN: 1932-6203
Popis: In the vertebrate embryo, the kidney is derived from the intermediate mesoderm. The LIM-class homeobox transcription factor lhx1 is expressed early in the intermediate mesoderm and is one of the first genes to be expressed in the nephric mesenchyme. In this study, we investigated the role of Lhx1 in specification of the kidney field by either overexpressing or depleting lhx1 in Xenopus embryos or depleting lhx1 in an explant culture system. By overexpressing a constitutively-active form of Lhx1, we established its capacity to expand the kidney field during the specification stage of kidney organogenesis. In addition, the ability of Lhx1 to expand the kidney field diminishes as kidney organogenesis transitions to the morphogenesis stage. In a complimentary set of experiments, we determined that embryos depleted of lhx1, show an almost complete loss of the kidney field. Using an explant culture system to induce kidney tissue, we confirmed that expression of genes from both proximal and distal kidney structures is affected by the absence of lhx1. Taken together our results demonstrate an essential role for Lhx1 in driving specification of the entire kidney field from the intermediate mesoderm.
Databáze: OpenAIRE
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