Effects of caffeine on behavioral and inflammatory changes elicited by copper in zebrafish larvae: Role of adenosine receptors

Autor: Carla Denise Bonan, Carlos Eduardo Leite, Maurício Reis Bogo, Fernanda Bueno Morrone, Fernanda Fernandes Cruz, Maria M. Campos, Giovanna Medeiros Tavares de Oliveira, Luiza Wilges Kist
Rok vydání: 2016
Předmět:
0301 basic medicine
Physiology
Health
Toxicology and Mutagenesis
Toxicology
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
Gene expression
Zebrafish
caffeine
Behavior
Animal
Gene Expression Regulation
Developmental
General Medicine
Larva
Tumor necrosis factor alpha
medicine.symptom
Inflammation Mediators
Caffeine
medicine.medical_specialty
Copper Sulfate
chemistry.chemical_element
Inflammation
Biology
Dinoprostone
03 medical and health sciences
Internal medicine
medicine
Purinergic P1 Receptor Agonists
Animals
Survival analysis
Osmolar Concentration
Receptors
Purinergic P1
Cell Biology
Zebrafish Proteins
biology.organism_classification
zebrafish
Adenosine receptor
Copper
Survival Analysis
030104 developmental biology
Endocrinology
chemistry
Purinergic P1 Receptor Antagonists
inflammation
030217 neurology & neurosurgery
Biomarkers
Water Pollutants
Chemical
Zdroj: Repositório Institucional PUCRS
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
ISSN: 1532-0456
Popis: This study investigated the effects of caffeine in the behavioral and inflammatory alterations caused by copper in zebrafish larvae, attempting to correlate these changes with the modulation of adenosine receptors. To perform a survival curve, 7dpf larvae were exposed to 10μM CuSO4, combined to different concentrations of caffeine (100μM, 500μM and 1mM) for up to 24h. The treatment with copper showed lower survival rates only when combined with 500μM and 1mM of caffeine. We selected 4 and 24h as treatment time-points. The behavior evaluation was done by analyzing the traveled distance, the number of entries in the center, and the length of permanence in the center and the periphery of the well. The exposure to 10μM CuSO4 plus 500μM caffeine at 4 and 24h changed the behavioral parameters. To study the inflammatory effects of caffeine, we assessed the PGE2 levels by using UHPLC-MS/MS, and TNF, COX-2, IL-6 and IL-10 gene expression by RT-qPCR. The expression of adenosine receptors was also evaluated with RT-qPCR. When combined to copper, caffeine altered inflammatory markers depending on the time of exposure. Adenosine receptors expression was significantly increased, especially after 4h exposure to copper and caffeine together or separately. Our results demonstrated that caffeine enhances the inflammation induced by copper by decreasing animal survival, altering inflammatory markers and promoting behavioral changes in zebrafish larvae. We also conclude that alterations in adenosine receptors are related to those effects.
Databáze: OpenAIRE
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