Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with freshly isolated gametes
| Autor: | Adele De Palma, Placido Borzì, Cinzia Di Pietro, Michele Purrello, Maria Rosa Guglielmino, Laura R Duro, Elisa Minutolo, Paolo Scollo, Marilena Vento, Alessandra Majorana, Davide Barbagallo, Marco Ragusa, Manuela Santonocito, M. R. Garofalo |
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| Rok vydání: | 2010 |
| Předmět: |
RNA Stability
Embryonic Development Cell Separation Validation Studies as Topic Biology Cryopreservation Transcriptome medicine Humans Vitrification Cells Cultured human oocytes Gene Expression Profiling Embryogenesis Obstetrics and Gynecology Oocyte Molecular biology vitrification Cell biology Gene expression profiling Reverse transcription polymerase chain reaction Germ Cells medicine.anatomical_structure Reproductive Medicine Oocytes gene expression Gamete Female |
| Zdroj: | Fertility and Sterility. 94:2804-2807 |
| ISSN: | 0015-0282 |
| Popis: | To assess the effects of vitrification on the biomolecular profile of oocytes, we analyzed through real-time reverse transcriptase-polymerase chain reaction eight genes encoding critically important proteins for embryo development and compared this partial transcriptome with that of freshly collected gametes isolated from the same women. The comparison of the molecular profiles demonstrated that our vitrification protocol does not alter the biomolecular quality of oocytes: in fact, between the two groups we found the absence of statistically significant variations. Accordingly, this cryopreservation technique might be helpful in preserving women's fertility. |
| Databáze: | OpenAIRE |
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