Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila
| Autor: | Eugene Schuster, Barbara H. Jennings, Robert F. Harvey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Chromosomal Proteins Non-Histone DNA transcription Polycomb-Group Proteins lcsh:Medicine RNA polymerase II Biology 03 medical and health sciences Transcription Elongation Factor SPT5 Model Organisms Molecular cell biology Suppression Genetic Genome Analysis Tools Gene expression Genetics Transcriptional regulation Animals Drosophila Proteins Wings Animal P-TEFb lcsh:Science Gene 030304 developmental biology Regulation of gene expression 0303 health sciences Binding Sites Multidisciplinary Drosophila Melanogaster 030302 biochemistry & molecular biology lcsh:R Genomics Animal Models Genome Scans 3. Good health Gene Expression Regulation biology.protein Female lcsh:Q Gene Function Transcriptional Elongation Factors Drosophila Protein Research Article Developmental Biology Protein Binding |
| Zdroj: | PLoS ONE, Vol 8, Iss 7, p e70184 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The early elongation checkpoint regulated by Positive Transcription Elongation Factor b (P-TEFb) is a critical control point for the expression of many genes. Spt5 interacts directly with RNA polymerase II and has an essential role in establishing this checkpoint, and also for further transcript elongation. Here we demonstrate that Drosophila Spt5 interacts both physically and genetically with the Polycomb Group (PcG) protein Pleiohomeotic (Pho), and the majority of Pho binding sites overlap with Spt5 binding sites across the genome in S2 cells. Our results indicate that Pho can interact with Spt5 to regulate transcription elongation in a gene specific manner. |
| Databáze: | OpenAIRE |
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