Expression of Cathepsin L and Its Intrinsic Inhibitors in Glomeruli of Rats With Puromycin Aminonucleoside Nephrosis
| Autor: | Kazumi Ishidoh, Yu Sasaki, Miyuki Takagi, Ayano Kubo, Isao Shirato, Katsuhiko Asanuma, Yusuke Suzuki, Teruo Hidaka |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Nephrotic Syndrome Histology Cathepsin L Nephrosis Kidney Glomerulus Puromycin Aminonucleoside urologic and male genital diseases Podocyte Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine Animals Cystatin B Cystatin C Cathepsin biology Podocytes Chemistry Glomerulonephritis Articles medicine.disease Immunohistochemistry Molecular biology Rats Disease Models Animal Proteinuria 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Cystatin Anatomy |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 66:863-877 |
| ISSN: | 1551-5044 0022-1554 |
| Popis: | Cathepsin L, a lysosomal cysteine proteinase, may have a key role in various biological and disease processes by intracellular and extracellular degradation of proteins. We examined the levels of cathepsin L and its intrinsic inhibitors in glomeruli of rats with puromycin aminonucleoside (PAN) nephrosis. In contrast to the weak levels of cathepsin L in normal glomeruli, on days 4 and 8, strong immunostaining was detected in almost all podocytes when proteinuria and pathological changes of the podocytes developed. Cathepsin L was reduced after day 28, but remained in a focal and segmental manner. Cystatin β, an intracellular inhibitor, was not detected in podocytes. However, cystatin C, an extracellular inhibitor, was detected in podocytes after day 4, coincident with cathepsin L. Cystatin C levels were gradually reduced but sustained in many podocytes on day 28, while cystatin C was not detected in podocytes sustained cathepsin L. These results demonstrated that cathepsin L levels are not always accompanied by the levels of its inhibitors in podocytes of PAN nephrosis, suggesting a potential role of cathepsin L in podocyte injury, which is a critical process for the development and progression of tuft adhesion and sclerosis. |
| Databáze: | OpenAIRE |
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