Partial immunological characterization of heat-stable proteinases fromPseudomonasspp. of dairy origin
| Autor: | Bernabé Sanz, Pablo E. Hernández, Rosario Martín, J. I. Azcona |
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| Rok vydání: | 1989 |
| Předmět: |
Antiserum
Immunodiffusion Hot Temperature biology Immune Sera Pseudomonas Proteolytic enzymes Enzyme-Linked Immunosorbent Assay Pseudomonas fluorescens Raw milk Precipitin biology.organism_classification Applied Microbiology and Biotechnology Microbiology Chromatography Affinity Milk Biochemistry Endopeptidases Pseudomonadales Food Microbiology Animals Enzyme Inhibitors Pseudomonadaceae |
| Zdroj: | Journal of Applied Bacteriology. 66:227-233 |
| ISSN: | 0021-8847 |
| DOI: | 10.1111/j.1365-2672.1989.tb02473.x |
| Popis: | A homogeneous extracellular heat-stable proteinase from Pseudomonas fluorescens AH-70 was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relationship of the proteinases from 26 psychrotrophic Pseudomonas spp. isolated from raw milk. The inhibition of the proteinases by the antiserum, the gel precipitin reactions and the use of a double-antibody sandwich ELISA, revealed that proteinase AH-70 is immunologically related to proteinases from 8/26 other Pseudomonas strains. These results also indicate that the immunological techniques for the detection of proteolytic enzymes in raw milk require antibody preparations of broader specificity. |
| Databáze: | OpenAIRE |
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