Inducible Capillary Formation in Lymphatic Endothelial Cells by Blocking Lipid Phosphate Phosphatase-3 Activity
| Autor: | Shunsuke Suzuki, Hiroaki Sakurai, Yoshiaki Tabuchi, Yoshinori Doki, Takayuki Minami, Takuro Misaki, Keiichi Koizumi, Orawin Prangsaengtong, Ikuo Saiki, Ichiro Takasaki, Kazutaka Senda |
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| Rok vydání: | 2009 |
| Předmět: |
government.form_of_government
Phosphatase Phosphatidate Phosphatase Biology Dephosphorylation Cell Adhesion Animals Humans RNA Messenger Lymphangiogenesis RNA Small Interfering Cell adhesion Lung Cells Cultured Lymphatic Vessels Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Endothelial Cells Dermis Capillaries Rats Cell biology Endothelial stem cell Lymphatic Endothelium Lymphatic system Biochemistry Vascular endothelial growth factor C government Endothelium Lymphatic Cardiology and Cardiovascular Medicine Biomarkers |
| Zdroj: | Lymphatic Research and Biology. 7:69-74 |
| ISSN: | 1557-8585 1539-6851 |
| DOI: | 10.1089/lrb.2009.0005 |
| Popis: | Lymphangiogenesis plays critical roles under normal and/or pathological conditions; however, the molecular contributors to this event were unknown until recently. In the present study, we first employed gene chip analysis and confirmed that lipid phosphate phosphatase-3 (LPP3) expression was increased until capillary formation in the conditionally immortalized rat lymphatic endothelial cell line. Signaling responses occur when several lipids induce acute biological functions; further, lipid phosphate phosphatases (LPPs) control their functions via dephosphorylation; however, there is no report on the association between LPP3 and lymphangiogenesis. siRNA-targeted LPP3 significantly increased capillary formation of human lymphatic endothelial cells; in contrast, it decreased cell adhesion to the basement membrane matrix. Furthermore, the inducible effect of the LPP inhibitor on capillary formation was observed. For the first time, we report that LPP3 abolishes accelerated abnormal lymphangiogenesis. Blocking LPP3 activities may aid in the development of novel therapy for lymph vessel defects. |
| Databáze: | OpenAIRE |
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