Human Herpes Simplex Virus-1 depletes APOBEC3A from nuclei
| Autor: | Ashok S. Bhagwat, Thomas C. Holland, Jessica A. Stewart |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cytoplasm
Recombinant Fusion Proteins viruses Green Fluorescent Proteins Herpesvirus 1 Human Biology medicine.disease_cause Article Virus Gene product Viral Proteins 03 medical and health sciences Cytidine Deaminase Virology Chlorocebus aethiops Gene expression medicine Animals Humans Immunologic Factors Vero Cells Gene 030304 developmental biology Cell Nucleus 0303 health sciences Staining and Labeling 030302 biochemistry & molecular biology Proteins Epstein–Barr virus Titer Viral replication Cell culture HeLa Cells |
| Zdroj: | Virology |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2019.08.012 |
| Popis: | APOBEC3 family of DNA-cytosine deaminases inactivate and mutate several human viruses. We constructed a human cell line that is inducible for EGFP-tagged APOBEC3A and found A3A predominantly in the nuclei. When these cells were infected with Herpes Simplex Virus-1, virus titer was unaffected by A3A expression despite nuclear virus replication. When A3A expression and virus infection were monitored, A3A was found predominantly to be nuclear in infected cells up to 3 hours post-infection, but was predominantly cytoplasmic by 12 hours. This effect did not require the whole virus, and could be reproduced using the UL39 gene of the virus which codes for a subunit of the viral ribonucleotide reductase. These results are similar to the reported exclusion of APOBEC3B by Epstein Barr virus ortholog of UL39, BORF2, but HSV1 UL39 gene product appears better at excluding A3A than A3B from nuclei. |
| Databáze: | OpenAIRE |
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