Vasomotor sympathetic neurons are more excitable than secretomotor sympathetic neurons in bullfrog paravertebral ganglia
| Autor: | John P. Horn, Paul H. M. Kullmann |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Patch-Clamp Techniques
Action Potentials Secretomotor Biology Article Sodium Channels Cellular and Molecular Neuroscience medicine Animals Patch clamp B cell Neurons Ganglia Sympathetic Rana catesbeiana Endocrine and Autonomic Systems Sodium channel Excitatory Postsynaptic Potentials Resting potential Paravertebral ganglia Vasomotor System medicine.anatomical_structure Excitatory postsynaptic potential Biophysics Neurology (clinical) Neuron Neuroscience |
| Zdroj: | Autonomic Neuroscience. 155:19-24 |
| ISSN: | 1566-0702 |
| Popis: | We compared the excitability of secretomotor B and vasomotor C neurons using virtual nicotinic synapses implemented with the dynamic clamp technique. In response to fast synaptic conductance (gsyn) waveforms modeled after B cell synaptic currents, it took 17.1 ± 1.2 nS to elicit spikes in 104 B cells and 3.3 ± 0.3 nS in 35 C cells. After normalizing for whole cell capacitance, C cells were still more excitable than B cells (76 ± 5 pS/pF vs. 169 ± 8 pS/pF). Stimulating C cells with slower gsyn waveforms, identical to synaptic currents in C cells, further accentuated the difference between cell types. The phentoypic excitability difference did not correlate with time in culture (1–12 days) and could not be explained by resting potential (B cells: −65.6 ± 0.9 mV, C cells: −63.1 ± 1.6 mV) or input conductance density, which was greater in C cells (24.4 ± 4.3 pS/pF) than B cells (14.5 ± 1.5 pS/pF). Action potentials elicited by virtual EPSPs had a threshold voltage for firing that was −28.4 ± 0.7 mV in C cells and −19.7 ± 0.4 mV B cells, and an upstroke velocity and peak spike potential that were greater in B cells. The repetitive firing properties of B and C cells were similar; 69–78 % phasic, 11–16 % adapting and 11–15 % tonic. We propose that B and C neurons express different types of Na+ channels that shape how they integrate nicotinic synaptic potentials. |
| Databáze: | OpenAIRE |
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