A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma

Autor: Patricia Baré, Fabian Fay, Cristina Alonso, Manuel Mendizabal, Cristian Oscar Rohr, Marcelo Silva, Federico Piñero, Martin P. Vazquez, Mariela Sciara
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Liver Cirrhosis
Male
Cirrhosis
Specialties of internal medicine
Ciencias de la Salud
INFLAMMATORY PATHWAYS
Gastroenterology
Feces
0302 clinical medicine
Prevotella
Prospective Studies
LIVER CANCER
biology
Microbiota
Liver Neoplasms
General Medicine
Middle Aged
Magnetic Resonance Imaging
MICROBIOTA
Otras Ciencias de la Salud
RC581-951
030220 oncology & carcinogenesis
Hepatocellular carcinoma
Biomarker (medicine)
Cytokines
Portal hypertension
purl.org/becyt/ford/3 [https]
030211 gastroenterology & hepatology
Female
medicine.symptom
Liver cancer
medicine.medical_specialty
Carcinoma
Hepatocellular
CIENCIAS MÉDICAS Y DE LA SALUD
Firmicutes
Inflammation
purl.org/becyt/ford/3.3 [https]
03 medical and health sciences
Internal medicine
medicine
Humans
Microbiome
Inflammatory pathways
neoplasms
Hepatology
business.industry
Lachnospiraceae
medicine.disease
biology.organism_classification
digestive system diseases
Gastrointestinal Microbiome
Case-Control Studies
Etiology
Tomography
X-Ray Computed
business
Follow-Up Studies
Zdroj: Annals of Hepatology, Vol 18, Iss 3, Pp 480-487 (2019)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Introduction and aim: A pro-oncogenic intestinal microbiome was observed in murine models; however, no specific microbiome in patients with hepatocellular carcinoma (HCC) has been reported. We aimed to compare the gut microbiome found in cirrhotic patients with or without HCC. Materials and methods: From 407 patients with Child Pugh A/B cirrhosis prospectively followed, 25 with HCC (cases) were matched with 25 without HCC (wo-HCC) in a 1:1 ratio according to age, gender, etiology, Child Pugh and severity of portal hypertension. In addition, results were also compared with 25 healthy subjects. Fecal stool samples were sequenced for the V3?V4 region of the microbial 16S rRNA (Illumina MiSeq Platform). Plasma cytokines were quantified including interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α). Results: We found a differential abundance in family members of Firmicutes with a 3-fold increase of Erysipelotrichaceae and a 5-fold decrease in family Leuconostocaceae in HCC when compared to wo-HCC controls. Genus Fusobacterium was found to be 5-fold decreased in HCC vs wo-HCC. The ratio bacteriodes/prevotella was increased in HCC. Three operational taxonomic units (OTUs), genus Odoribacter and Butyricimonas were more abundant in HCC, whereas a decreased abundance in Lachnospiraceae family genus Dorea was observed in HCC patients. A Random Forest model trained with differential abundant taxa correctly classified HCC individuals. This pattern was associated with an inflammatory milieu with a putative increased activation of NOD-like receptor pathways. Conclusion: We found a pattern of microbiome linked to inflammation that could be potentially useful as HCC biomarker after follow-up validation studies. Fil: Piñero, Federico. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina. Universidad Austral; Argentina Fil: Vazquez, Martín. Heritas; Argentina Fil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Rohr, Cristian Oscar. Heritas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mendizabal, Manuel. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina Fil: Sciara, Mariela Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Heritas; Argentina Fil: Alonso, Cristina. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina Fil: Fay, Fabián. Heritas; Argentina Fil: Silva, Marcelo. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina
Databáze: OpenAIRE
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