Pressure-Induced Changes in Astrocyte GFAP, Actin, and Nuclear Morphology in Mouse Optic Nerve
| Autor: | Harry A. Quigley, Mary Ellen Pease, Yik Tung Tracy Ling, Elizabeth C. Kimball, Thao D. Nguyen, Joan L. Jefferys |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
genetic structures Intermediate Filaments Glaucoma Ocular hypertension 03 medical and health sciences Mice 0302 clinical medicine Glial Fibrillary Acidic Protein medicine Animals Axon Intraocular Pressure Glial fibrillary acidic protein biology Chemistry GFAP nucleus astrocytes Optic Nerve optic nerve head General Medicine medicine.disease Actins eye diseases IOP elevation Cell nucleus Disease Models Animal 030104 developmental biology medicine.anatomical_structure Biophysics Optic nerve biology.protein sense organs Erratum Nucleus actin 030217 neurology & neurosurgery Astrocyte |
| Zdroj: | Investigative Ophthalmology & Visual Science |
| ISSN: | 1552-5783 0146-0404 |
| Popis: | Purpose To conduct quantitative analysis of astrocytic glial fibrillary acidic protein (GFAP), actin and nuclei distribution in mouse optic nerve (ON) and investigate changes in the measured features after 3 days of ocular hypertension (OHT). Method Serial cross-sections of 3-day microbead-induced OHT and control ONs were fluorescently labelled and imaged using confocal microscope. Eighteen structural features were measured from the acquired images, including GFAP coverage, actin area fraction, process thickness, and aspect ratio of cell nucleus. The measured features were analyzed for variations with axial locations along ON and radial zones transverse to ON, as well as for the correlations with degree of intraocular pressure (IOP) change. Results The most significant changes in structural features after 3-day OHT occurred in the unmyelinated ON region (R1), and the changes were greater with greater IOP elevation. Although the GFAP, actin, axonal, and ON areas all increased in 3-day OHT ONs in R1 (P ≤ 0.004 for all), the area fraction of GFAP actually decreased (P = 0.02), the actin area fraction was stable and individual axon compartments were unchanged in size. Within R1, the number of nuclear clusters increased (P < 0.001), but the mean size of nuclear clusters was smaller (P = 0.02) and the clusters became rounder (P < 0.001). In all cross-sections of control ONs, astrocytic processes were thickest in the rim zone compared with the central and peripheral zones (P ≤ 0.002 for both), whereas the overall process width in R1 decreased after 3 days of OHT (P < 0.001). Conclusions The changes in structure elucidated IOP-generated alterations that underlie astrocyte mechanotranslational responses relevant to glaucoma. |
| Databáze: | OpenAIRE |
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