Oct-4 regulates the expression of Stella and Foxj2 at the Nanog locus: implications for the developmental competence of mouse oocytes
| Autor: | Michele Bellone, Lucia Sacchi, Valeria Merico, Silvia Garagna, Carlo Alberto Redi, Mario Stefanelli, Maurizio Zuccotti, Riccardo Bellazzi, Thore C. Brink, James Adjaye |
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| Rok vydání: | 2009 |
| Předmět: |
Homeobox protein NANOG
Chromosomal Proteins Non-Histone Nucleolus Germ cell nuclear factor Down-Regulation Biology Oct-4 Mice Oogenesis medicine Animals Homeodomain Proteins Genetics Rehabilitation Obstetrics and Gynecology Nanog Homeobox Protein Forkhead Transcription Factors Oocyte Up-Regulation Chromatin Cell biology Repressor Proteins medicine.anatomical_structure Reproductive Medicine Oocytes Female Folliculogenesis Octamer Transcription Factor-3 |
| Zdroj: | Human Reproduction. 24:2225-2237 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/dep191 |
| Popis: | Background Our knowledge of what determines the mammalian oocyte developmental competence is meagre. By comparing the transcriptional profiles of developmentally competent surrounded nucleolus (SN) and incompetent not surrounded nucleolus (NSN) mouse MII oocytes, we recently demonstrated that Oct-4 and Stella are key factors in the establishment of the oocytes' developmental competence. Methods Using RT-PCR, microarray and immunocytochemistry assays, we analysed expression of genes and proteins in oocytes isolated throughout folliculogenesis and classified based on their SN- or NSN-type of chromatin organization. Results We show that: (1) Oct-4 and Stella are expressed concurrently at the beginning of oocytes' growth and only in SN oocytes; (2) Germ Cell Nuclear Factor is a putative regulator of Oct-4 expression in MII oocytes; (3) the function of Oct-4 is directed at the Nanog locus, regulating the expression of Stella and Foxj2. Conclusions (1) A number of factors that act upstream and downstream of Oct-4 emerge as candidate players in the acquisition of the oocyte's developmental competence; (2) we define molecular markers that identify a specific group of ovarian oocytes (SN) that have a potential to acquire developmental competence; (3) the presence of SN and NSN oocytes in human ovaries extends the interest of these results to the field of human reproduction. |
| Databáze: | OpenAIRE |
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