Stanozolol administration combined with exercise leads to decreased telomerase activity possibly associated with liver aging
| Autor: | Mehtap Kara, Aristides M. Tsatsakis, Eren Ozcagli, Polychronis Stivaktakis, Tuğba Kotil, Persefoni Fragkiadaki, Dimitrios Tsoukalas, Christina Tsitsimpikou, Manolis Tzatzarakis, Demetrios A. Spandidos, Buket Alpertunga |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
phosphatase and tensin homolog Male medicine.medical_specialty Telomerase Aging Anabolic Agents Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine 3′-hydroxystanozolol Internal medicine Physical Conditioning Animal Genetics medicine Tensin PTEN Animals Telomerase reverse transcriptase Stanozolol Testosterone biology business.industry telomerase reverse transcriptase PTEN Phosphohydrolase telomerase activity General Medicine Articles Telomere stanozolol Molecular medicine Immunohistochemistry 030104 developmental biology Endocrinology Gene Expression Regulation Liver biology.protein Metabolome business 16-β-hydroxystanozolol 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | Anabolic agents are doping substances which are commonly used in sports. Stanozolol, a 17α-alkylated derivative of testosterone, has a widespread use among athletes and bodybuilders. Several medical and behavioral adverse effects are associated with anabolic androgenic steroids (AAS) abuse, while the liver remains the most well recognized target organ. In the present study, the hepatic effects of stanozolol administration in rats at high doses resembling those used for doping purposes were investigated, in the presence or absence of exercise. Stanozolol and its metabolites, 16-β-hydroxystanozolol and 3′-hydroxystanozolol, were detected in rat livers using liquid chromatography-mass spectrometry (LC-MS). Telomerase activity, which is involved in cellular aging and tumorigenesis, was detected by examining telomerase reverse transcriptase (TERT) and phosphatase and tensin homolog (PTEN) expression levels in the livers of stanozolol-treated rats. Stanozolol induced telomerase activity at the molecular level in the liver tissue of rats and exercise reversed this induction, reflecting possible premature liver tissue aging. PTEN gene expression in the rat livers was practically unaffected either by exercise or by stanozolol administration. |
| Databáze: | OpenAIRE |
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