Anaerobic biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by Acetobacterium malicum strain HAAP-1 isolated from a methanogenic mixed culture
| Autor: | Neal R. Adrian, Clint M. Arnett |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Electron donor Biology Applied Microbiology and Biotechnology Microbiology DNA Ribosomal Acetobacterium chemistry.chemical_compound Bacterial Proteins Formaldehyde RNA Ribosomal 16S Sequence Homology Nucleic Acid Anaerobiosis Biomass Phylogeny Triazine Acetic Acid Acetobacterium malicum Chromatography Strain (chemistry) Triazines Genes rRNA General Medicine Sequence Analysis DNA Biodegradation 16S ribosomal RNA Biodegradation Environmental chemistry Degradation (geology) Anaerobic exercise Oxidation-Reduction Hydrogen |
| Zdroj: | Current microbiology. 48(5) |
| ISSN: | 0343-8651 |
| Popis: | In previous work, we studied the anaerobic biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by a methanogenic mixed culture that biodegrades RDX by using H2 as the sole electron donor. Strain HAAP-1 was isolated after enriching for the homoacetogens in a mineral medium containing RDX and an H2-CO2 (80:20) headspace. Strain HAAP-1 degraded 29.0 microM RDX in14 days and formed 13.0 mM acetate when grown in a mineral medium with an H2-CO2 headspace. Methylenedinitramine was observed as a transient intermediate, indicating ring cleavage had occurred. In live cultures containing an N2-CO2 headspace, RDX was not degraded, and no acetate was formed. The 16S rRNA gene sequence for strain HAAP-1, consisting of 1485 base pairs, had a 99.2% and 99.1% sequence similarity to Acetobacterium malicum and A. wieringae, respectively. This is the first report of RDX degradation by a homoacetogen growing autotrophically and extends the number of genera known to carry out this transformation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink