Neuronal Cell Surface Molecules Mediate Specific Binding to Rabies Virus Glycoprotein Expressed by a Recombinant Baculovirus on the Surfaces of Lepidopteran Cells
| Autor: | Mark C. Fishman, Anne-Marie Roque Alfonso, Christine Tuffereau, Anne Flamand, Jacqueline Benejean |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cell type
Immunology DNA Recombinant Nerve Tissue Proteins Receptors Cell Surface Plasma protein binding Spodoptera medicine.disease_cause Microbiology Cell Line Mice Neuroblastoma Viral Proteins Virology medicine Animals Glycoproteins Sf21 Neurons Binding Sites biology Rabies virus biology.organism_classification Molecular biology Virus-Cell Interactions Cell culture Vesicular stomatitis virus Insect Science biology.protein Receptors Virus Antibody Baculoviridae Protein Binding |
| Zdroj: | Journal of Virology. 72:1085-1091 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.72.2.1085-1091.1998 |
| Popis: | The existence of specific rabies virus (RV) glycoprotein (G) binding sites on the surfaces of neuroblastoma cells is demonstrated. Spodoptera frugiperda (Sf21) cells expressing G of the RV strain CVS (Gcvs-Sf21 cells) bind specifically to neuroblastoma cells of different species but not to any other cell type (fibroblast, myoblast, epithelial, or glioma). Attachment to mouse neuroblastoma NG108-15 cells is abolished by previous treatment of Gcvs-Sf21 cells with anti-G antibody. Substitutions for lysine at position 330 and for arginine at position 333 in RV G greatly reduce interaction between Gcvs-Sf21 cells and NG108-15 cells. These data are consistent with in vivo results: an avirulent RV mutant bearing the same double mutation is not able to infect sensory neurons or motoneurons (P. Coulon, J.-P. Ternaux, A. Flamand, and C. Tuffereau, J. Virol. 72:273–278, 1998) after intramuscular inoculation into a mouse. Furthermore, infection of NG108-15 cells by RV but not by vesicular stomatitis virus leads to a reduction of the number of binding sites at the neuronal-cell surface. Our data strongly suggest that these specific attachment sites on neuroblastoma cells represent a neuronal receptor(s) used by RV to infect certain types of neurons in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|