Luminescent lanthanide-binding peptides: sensitising the excited states of Eu(iii ) and Tb(iii ) with a 1,8-naphthalimide-based antenna
| Autor: | Célia S. Bonnet, Thorfinnur Gunnlaugsson, Marc Devocelle |
|---|---|
| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Physiology and Medical Physics, School of Biochemistry and Immunology, Trinity College Dublin |
| Rok vydání: | 2012 |
| Předmět: |
Lanthanide
Magnetic Resonance Spectroscopy MESH: Europium MULTIWAVELENGTH SPECTROSCOPIC DATA chemistry.chemical_element Terbium Peptide 010402 general chemistry Photochemistry Lanthanoid Series Elements 01 natural sciences Biochemistry AQUEOUS-SOLUTION HIGH-AFFINITY Europium CHARGE-TRANSFER MESH: Lanthanoid Series Elements MESH: Terbium AMINO-ACIDS [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Physical and Theoretical Chemistry chemistry.chemical_classification METAL-ION BINDING MESH: Magnetic Resonance Spectroscopy MESH: Peptides 010405 organic chemistry Chemistry fungi Organic Chemistry TRYPTOPHAN-CONTAINING PARVALBUMIN Nuclear magnetic resonance spectroscopy Chromophore Amides MESH: Amides 0104 chemical sciences MESH: Luminescent Measurements Excited state Luminescent Measurements CALCIUM-BINDING EQUILIBRIUM-CONSTANTS Peptides ENERGY-TRANSFER Luminescence |
| Zdroj: | Organic and Biomolecular Chemistry Organic and Biomolecular Chemistry, Royal Society of Chemistry, 2012, 10 (1), pp.126-33. ⟨10.1039/c1ob06567j⟩ |
| ISSN: | 1477-0539 1477-0520 |
| DOI: | 10.1039/c1ob06567j |
| Popis: | International audience; The investigation into the luminescence properties of a lanthanide-binding peptide, derived from the Ca-binding loop of the parvalbumin, and modified by incorporating a 1,8-naphthalimide (Naph) chromophore at the N-terminus is described. Here, the Naph is used as a sensitising antenna, which can be excited at lower energy than classical aromatic amino acids, such as tryptophan (the dodecapeptide of which was also synthesised and studied herein). The syntheses of the Naph antenna, its solid phase incorporation into the dodecapeptide, and the NMR investigation into the formation of the corresponding lanthanide complexes in solution is presented. We also show that this Naph antenna can be successfully employed to sensitize the excited states of both europium and terbium ions, the results of which was used to determined the stability constants of their formation complexes, and we demonstrated that our peptide 'loop' can selectively bind these lanthanide ions over Ca(II). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|