Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry
Autor: | Pavol Ďurč, Wolfgang Buchberger, Štefan Konečný, Věra Dosedělová, Jiří Dolina, Markus Himmelsbach, Petr Kubáň, František Foret |
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Rok vydání: | 2020 |
Předmět: |
Adult
Male Saliva Spectrometry Mass Electrospray Ionization Formic acid Electrospray ionization Pilot Projects 010402 general chemistry 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry Bile Acids and Salts chemistry.chemical_compound Barrett Esophagus medicine Glycochenodeoxycholic acid Humans Exhaled breath condensate Esophagus Chromatography High Pressure Liquid Principal Component Analysis Chromatography 010401 analytical chemistry Organic Chemistry Reproducibility of Results Signal Processing Computer-Assisted General Medicine Middle Aged Reference Standards medicine.disease digestive system diseases 3. Good health 0104 chemical sciences medicine.anatomical_structure chemistry Barrett's esophagus Female |
Zdroj: | Journal of chromatography. A. 1625 |
ISSN: | 1873-3778 |
Popis: | A fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease. |
Databáze: | OpenAIRE |
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