Validation of an Analytical Method for the Determination of Pesticide Residues in Vine Leaves by GC-MS/MS
| Autor: | Marivil Islam, Andrew Cannavan, Amer Abu Alnaser, Britt Maestroni, Iyad Ghanem, Simon Kelly, Verónica Cesio, Horacio Heinzen |
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| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Chromatography Gas Chromatography Pesticide residue 010401 analytical chemistry Pesticide Residues Ethyl acetate General Chemistry Repeatability 010501 environmental sciences Pesticide Quechers Sensitivity and Specificity 01 natural sciences 0104 chemical sciences Plant Leaves chemistry.chemical_compound chemistry Tandem Mass Spectrometry Vitis Gas chromatography Gas chromatography–mass spectrometry General Agricultural and Biological Sciences 0105 earth and related environmental sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 66:6421-6430 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.8b00453 |
| Popis: | A modified and miniaturized SweEt/QuEChERS method for pesticide residue analysis applied to vine leaves is presented. The deep-frozen plant material was cryogenically processed. A 2 g analytical portion was hydrated for 30 min and extracted with acidified ethyl acetate after buffering with NaHCO3 and adding Na2SO4. A dispersive solid-phase (d-SPE) cleanup step with primary-secondary amine (PSA) was performed. The pesticide residues were determined using GC-MS/MS. The whole procedure was validated for 54-59 pesticides at 0.01, 0.02, 0.1, and 0.2 mg/kg in fresh vine leaves ( Vitis vinifera). The key method performance parameters investigated were specificity, linearity, trueness, within laboratory repeatability and reproducibility, limit of detection, limit of quantitation, and matrix effects. Recoveries for the 59 pesticides tested ranged from 60 to 110%, and the RSDs were lower than 20% for the majority of the pesticides studied. |
| Databáze: | OpenAIRE |
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