Ouabain pre-treatment modulates B and T lymphocytes and improves survival of melanoma-bearing animals
| Autor: | Maria Luisa Arantes Campos, Raul Correia Aleixo, Mariana Pires Teixeira, Vinicius Ribeiro Cabral, Felipe Jeová Pereira Cavalcante, Augusto das Neves Azevedo, Lucas Zanetti de Albuquerque, Renan Faustino, Lays Ribeiro Oliveira Gomes, Luciana Souza de Paiva, Joyle Moreira Carvalho da Silva |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Skin Neoplasms Regulatory T cell Lymphocyte Immunology Melanoma Experimental Antineoplastic Agents Spleen Pharmacology T-Lymphocytes Regulatory Ouabain Flow cytometry Immunomodulation Mice 03 medical and health sciences 0302 clinical medicine Immune system In vivo Animals Humans Immunology and Allergy Medicine Melanoma B-Lymphocytes medicine.diagnostic_test business.industry medicine.disease Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Female business Injections Intraperitoneal medicine.drug |
| Zdroj: | International Immunopharmacology. 86:106772 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2020.106772 |
| Popis: | Ouabain (OUA) is a glycoside shown to modulate B and T lymphocytes. Nevertheless, ouabain effects on B16F10 melanoma immune response, a mouse lineage that mimics human melanoma, are still unknown. Our aim was to study how OUA in vivo treatment modulates lymphocytes and if it improves the response against B16F10 cells. C57BL/6 mice were pre-treated with intraperitoneal (i.p) injection of OUA (0.56 mg/Kg) for three consecutive days. On the 4th day, 106 B16F10 cells or vehicle were i.p. injected. Animals were euthanized on days 4th and 21st for organs removal and subsequent lymphocyte analyses by flow cytometry. In vivo ouabain-treatment reduced regulatory T cells in the spleen in both melanoma and non-melanoma groups. Ouabain preserved the number and percentage of B lymphocytes in peripheral organs of melanoma-injected mice. Melanoma-injected mice pre-treated with OUA also survive longer. Our findings contribute to a better understanding of OUA immunological effects in a melanoma model. |
| Databáze: | OpenAIRE |
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