Expression of Adenine Nucleotide Translocator Correlates with the IL-4-induced lymphocyte survival: Role of PI3K and MAPK
| Autor: | Ji-Young Jang, Choong-Eun Lee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
MAPK/ERK pathway
biology medicine.diagnostic_test Chemistry lcsh:T Adenine nucleotide translocator Lymphocyte lcsh:R Short Report lcsh:Medicine General Medicine Molecular biology lcsh:Technology General Biochemistry Genetics and Molecular Biology Flow cytometry medicine.anatomical_structure Apoptosis biology.protein medicine DNA fragmentation lcsh:Q Northern blot lcsh:Science Interleukin 4 General Environmental Science |
| Zdroj: | The Scientific World Journal The Scientific World Journal, Vol 1, Pp 100-100 (2001) |
| ISSN: | 1537-744X |
| Popis: | MATERIALS AND METHODS. We have employed DD-PCR techniques to screen novel genes whose expression is differentially regulated by Th1 and Th2 cytokines in human lymphocytes. One of the selected DD-PCR products was adenine nucleotide translocator(ANT3). The mRNA expression pattern by various Th1 and Th2 cytokines was analyzed by Northern blot and RT-PCR in B, T, and monocytic cells. The apoptosis study was performed using DNA fragmentation analysis and DiOC6 and PI staining by flow cytometry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text