| Autor: |
Ya-Ting Zhang, Min Dong, Pan-Pan Xu, Jia-Hui Cai, Shi-He Liu, Ya-Biao Gao, Li-Bin Wang, Jian Li, Hong Jiang, Ji-Dong Wang, De-Song Wang |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Colloids and surfaces. B, Biointerfaces. 218 |
| ISSN: |
1873-4367 |
| Popis: |
Effectively capturing, releasing, and reanalyzing circulating tumor cells (CTCs) are critical in cancer diagnosis and individualized treatment. Traditional immunomagnetic separation has disadvantages of low sensitivity and specificity, and is time-consuming and costly in CTCs capture. It is also easily disturbed by the microenvironment in releasing and analyzing CTCs. Here, we proposed an aptamer-mediated DNA concatemer functionalized magnetic nanoparticles (MNPs-AMDC) for the reversible capture and release of CTCs. In this study, aptamers were used both for efficiently capturing CTCs without complicated assembly steps and stimulus-response switch for releasing CTCs with little influence on cellular activity. The MNPs-AMDC was demonstrated to effectively capture (83%) and release CTCs with a good viability rate (92%). Moreover, this device was also tested in clinical blood samples, which would provide a universal tool for diagnosing cancer and treating individuals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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