Exocytosis of MTT formazan could exacerbate cell injury
| Autor: | David T. Yew, Jie Xu, Maria Sen Mun Wai, Lihong Zhang, Lanhai Lü |
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| Rok vydání: | 2010 |
| Předmět: |
Programmed cell death
SH-SY5Y Formazans Microscopy Confocal Cell growth Caspase 3 Cell Survival Cell Tetrazolium Salts Apoptosis General Medicine Biology Toxicology Endocytosis Exocytosis Cell biology medicine.anatomical_structure Microscopy Electron Transmission Cell Line Tumor medicine Humans Viability assay |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 26(4) |
| ISSN: | 1879-3177 |
| Popis: | MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method is one of the most widely used methods to analyze cell proliferation and viability. It is taken up through endocytosis and is reduced by mitochondrial enzymes as well as endosomal/lysosomal compartments, then is transported to cell surfaces to form needle-like MTT formazans; however the effect of MTT itself still remains elusive. Our objective was to investigate the direct effects of MTT on in vitro SH-SY5Y cells. Results showed that the endocytosis of MTT did not cause obvious lesion and induce cell death, but the metabolism and exocytosis of MTT could dramatically damage cells. Our results also indicated that MTT could activate apoptosis related factors such as caspase-8, caspase-3 or accelerate the leakage of cell contents after the appearance of MTT formazan crystals. The present data suggest MTT method should be carefully chosen; otherwise the cell viability would be underestimated and incomparable. |
| Databáze: | OpenAIRE |
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