FTZ-F1-related orphan receptors in Xenopus laevis: transcriptional regulators differentially expressed during early embryogenesis

Autor: Heiko Keller, V Laudet, Walter Wahli, Christine Dreyer, H Ellinger-Ziegelbauer, A K Hihi
Rok vydání: 1994
Předmět:
Receptors
Steroid
DNA
Complementary
Embryo
Nonmammalian
Transcription
Genetic
Molecular Sequence Data
Restriction Mapping
Xenopus
Fushi Tarazu Transcription Factors
Gene Expression
Receptors
Cytoplasmic and Nuclear
Steroid biosynthesis
Steroidogenic Factor 1
Transfection
Mice
Xenopus laevis
Animals
Drosophila Proteins
Humans
Amino Acid Sequence
RNA
Messenger
Cloning
Molecular
Promoter Regions
Genetic
Receptor
Molecular Biology
Phylogeny
Homeodomain Proteins
Orphan receptor
Base Sequence
biology
Membrane Proteins
Embryo
Cell Biology
biology.organism_classification
Molecular biology
Globins
Neuron-derived orphan receptor 1
DNA-Binding Proteins
Drosophila melanogaster
Oligodeoxyribonucleotides
Neurula
Nuclear receptor
Insect Proteins
Cattle
Carrier Proteins
HeLa Cells
Transcription Factors
Research Article
Zdroj: Molecular and Cellular Biology. 14:2786-2797
ISSN: 1098-5549
0270-7306
DOI: 10.1128/mcb.14.4.2786
Popis: Orphan receptors of the FTZ-F1-related group of nuclear receptors (xFF1r) were identified in Xenopus laevis by isolation of cDNAs from a neurula stage library. Two cDNAs were found, which encode full length, highly related receptor proteins, xFF1rA and B, whose closet relative known so far is the murine LRH-1 orphan receptor. xFF1rA protein expressed by a recombinant vaccinia virus system specifically binds to FTZ-F1 response elements (FRE; PyCAAGGPyCPu). In cotransfection studies, xFF1rA constitutively activates transcription, in a manner dependent on the number of FREs. The amounts of at least four mRNAs encoding full-length receptors greatly increase between gastrula and early tailbud stages and decrease at later stages. At early tailbud stages, xFTZ-F1-related antigens are found in all nuclei of the embryo.
Databáze: OpenAIRE
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