Contribution of AIDA-I to the pathogenicity of a porcine diarrheagenic Escherichia coli and to intestinal colonization through biofilm formation in pigs
| Autor: | Dorothy M. Middleton, Carlton L. Gyles, Michael Mourez, Elemir Simko, Musangu Ngeleka, Frédéric Berthiaume, Sang-Hyun Kim, Madhu Ravi |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
Virulence medicine.disease_cause Polymerase Chain Reaction Microbiology Bacterial Adhesion Microscopy Electron Transmission In vivo Escherichia coli medicine Animals Humans Microscopy Immunoelectron Escherichia coli Infections Swine Diseases Adhesins Escherichia coli General Veterinary biology Strain (chemistry) Biofilm General Medicine Immunogold labelling biology.organism_classification Antibodies Bacterial Immunohistochemistry Enterobacteriaceae Intestines Bacterial adhesin Animals Newborn Biofilms Mutation HeLa Cells |
| Zdroj: | Veterinary Microbiology. 120:308-319 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2006.10.035 |
| Popis: | In order to evaluate the role of the AIDA-I of porcine diarrheagenic Escherichia coli strain PD20 serogroup O143 (AIDA-I(+), STb(+)), a mutant strain PD20M (AIDA-I(-), STb(+)) was generated from strain PD20 by an allelic exchange procedure. In addition, the full-length aidA gene was reintroduced into strain PD20M to generate the complemented strain PD20C (pTaidA, AIDA-I(+), STb(+)). A non-pathogenic E. coli strain PD71 was used as negative control. Each strain was inoculated to newborn pigs via stomach tube. Severity of diarrhea was evaluated clinically and intestinal colonization was assessed by histology, immunohistochemistry (IHC), and transmission electron microscopy (TEM) including immunogold electron microscopy (IGEM). The adhesion pattern to HeLa cells, bacterial auto-aggregation and biofilm formation were evaluated in vitro. Pigs infected with strains PD20 or PD20C developed diarrhea 16 and 28h after inoculation, respectively, in contrast to pigs infected with strains PD20M or PD71. Histology, IHC, TEM and IGEM examinations showed heavy bacterial colonization with biofilm formation in the large intestine, and marked in vivo expression of AIDA-I protein in pigs infected with strains PD20 or PD20C in contrast to pigs infected with strains PD20M or PD71. The in vitro assays showed marked diffuse adherence to HeLa cells, enhanced bacterial auto-aggregation and significant biofilm formation (p |
| Databáze: | OpenAIRE |
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