Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice
| Autor: | Soroush Darvish-Ghane, Min Zhuo, Manabu Yamanaka |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Aging evoked excitatory postsynaptic currents Dopamine AMPA/KA receptors Synaptic Transmission Receptors G-Protein-Coupled 0302 clinical medicine Receptors Kainic Acid Postsynaptic potential spontaneous excitatory postsynaptic currents Chemistry musculoskeletal neural and ocular physiology Pyramidal Cells Glutamate receptor Long-term potentiation Excitatory postsynaptic potential Molecular Medicine medicine.drug Signal Transduction Research Article medicine.medical_specialty AMPA receptor Tetrodotoxin Inhibitory postsynaptic potential Gyrus Cinguli Models Biological Anterior cingulate cortex 03 medical and health sciences Cellular and Molecular Neuroscience Internal medicine medicine Animals alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid D2 receptors Post-tetanic potentiation Receptors Dopamine D2 miniature excitatory postsynaptic currents Excitatory Postsynaptic Potentials Neural Inhibition Benzazepines Mice Inbred C57BL 030104 developmental biology Anesthesiology and Pain Medicine Endocrinology nervous system Sulpiride Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Molecular Pain |
| ISSN: | 1744-8069 |
| Popis: | Dopamine (DA) possesses potent neuromodulatory properties in the central nervous system. In the anterior cingulate cortex, α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPAR) are key ion channels in mediating nerve injury induced long-term potentiation (LTP) and chronic pain phenotype. In the present study, we reported the effects of DA on glutamate mediated excitatory post-synaptic currents (EPSCs) in pyramidal neurons of layer II/III of the ACC in adult mice. Bath application of DA (50 μM) caused a significant, rapid and reversible inhibition of evoked EPSCs (eEPSC). This inhibitory effect is dose-related and was absent in lower concentration of DA (5 μM). Furthermore, selective postsynaptic application of GDP-β-S (1.6 mM) in the internal solution completely abolished the inhibitory effects of DA (50 μM). We also investigated modulation of spontaneous EPSCs (sEPSCs) and TTX sensitive, miniature EPSCs (mEPSCs) by DA. Our results indicated mixed effects of potentiation and inhibition of frequency and amplitude for sEPSCs and mEPSCs. Furthermore, high doses of SCH23390 (100 μM) and sulpiride (100 μM) revealed that, inhibition of eEPSCs is mediated by postsynaptic D2-receptors (D2R). Our finding posits a pre- and postsynaptic mode of pyramidal neuron EPSC modulation in mice ACC by DA. |
| Databáze: | OpenAIRE |
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