NF-Y and USF1 transcription factor binding to CCAAT-box and E-box elements activates the CP27 promoter
| Autor: | Smit Dangaria, Xianghong Luan, Yoshihiro Ito, Youbin Zhang, Thomas G.H. Diekwisch |
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| Rok vydání: | 2011 |
| Předmět: |
Regulation of gene expression
Extracellular Matrix Proteins Base Sequence Response element CAAT box Nuclear Proteins Proteins Promoter General Medicine Biology Molecular biology Article Chromatin E-Box Elements Mice Epigenetics of physical exercise CCAAT-Binding Factor Gene Expression Regulation Genetics Animals Upstream Stimulatory Factors Promoter Regions Genetic Transcription factor Embryonic Stem Cells ChIA-PET |
| Zdroj: | Gene. 473:92-99 |
| ISSN: | 0378-1119 |
| Popis: | The maintenance and differentiation of embryonic stem cells (ES cells) depends on the regulation of gene expression through the coordinated binding of transcription factors to regulatory promoter elements. One of the genes involved in embryonic development is the chromatin factor CP27. Previously, we have shown that NF-Y interacted with the CP27 proximal promoter CCAAT-box. Here we report that CP27 gene expression in mouse ES cells is controlled by CCAAT and E-box cis-acting regulatory elements and their corresponding transcription factors NF-Y and USF1. Specifically, USF1 interacts with the E-box of the CP27 proximal promoter and NF-Y interacts with the CCAAT-box. NF-Y and USF1 also interacted with each other and activated the CP27 promoter in a synergistic fashion. Together, these studies demonstrate that gene expression of the chromatin factor CP27 is regulated through the interaction of the transcription factors NF-Y and USF1 with the CP27 proximal promoter. |
| Databáze: | OpenAIRE |
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