Plant-Derived Monoterpenes Suppress Hamster Kidney Cell 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Synthesis at the Post-Transcriptional Level
| Autor: | Apurba K. Gayen, Dennis M. Peffley |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
7-Dehydrocholesterol reductase
Translational efficiency Mevalonic Acid Medicine (miscellaneous) Reductase Kidney chemistry.chemical_compound Biosynthesis Cricetinae Animals Lovastatin Molecular Biology Cells Cultured Nutrition and Dietetics Mesocricetus biology Plant Extracts Cholesterol Perillyl alcohol Hydroxymethylglutaryl-CoA reductase chemistry Biochemistry HMG-CoA reductase Monoterpenes biology.protein Autoradiography RNA Hydroxymethylglutaryl CoA Reductases lipids (amino acids peptides and proteins) Hydroxymethylglutaryl-CoA Reductase Inhibitors |
| Zdroj: | The Journal of Nutrition. 133:38-44 |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/133.1.38 |
| Popis: | The rate-limiting enzyme for mevalonate and cholesterol synthesis in mammalian cells is 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase. Control occurs through both transcriptional and post-transcriptional actions signaled by the end product, cholesterol, and by isoprenoid intermediates. End products of plant mevalonate metabolism, i.e., plant-derived isoprenoids, also suppress mammalian HMG-CoA reductase. Previous studies reported that isoprenoids suppress reductase synthesis at a post-transcriptional level. We tested the hypothesis that plant-derived isoprenoids also regulate mammalian HMG-CoA reductase synthesis at a post-transcriptional level by incubating lovastatin-treated C100 cells with mevalonate or a plant-derived isoprenoid (the monoterpenes, limonene, perillyl alcohol or geraniol) either alone or combined with the oxysterol, 25-hydroxycholesterol (25-OH C). Mevalonate decreased HMG-CoA reductase synthesis and mRNA levels by 65 and 66%, respectively (P < 0.05). The cyclic monoterpenes, limonene and perillyl alcohol, lowered HMG-CoA reductase synthesis by 70 and 89%, respectively (P < 0.05); although neither reduced HMG-CoA reductase mRNA levels (P = 0.88). Geraniol, an acyclic monoterpene, suppressed HMG-CoA reductase synthesis by 98% and lowered mRNA levels by 66% (P < 0.05). A combination of 25-OH C and either mevalonate or any three monoterpenes reduced HMG-CoA reductase mRNA levels (P < 0.05) compared with lovastatin-only treated cells. However, the dual combination of 25-OH C and either mevalonate or a monoterpene resulted in a greater decrease in HMG-CoA reductase synthesis than in mRNA levels. The difference between changes in HMG-CoA reductase synthesis and mRNA levels reflects a specific effect of isoprenoids on HMG-CoA reductase synthesis at the translational level. Mevalonate enhanced HMG-CoA reductase degradation, but no such effect was observed for the monoterpenes. These results indicate that the three plant-derived isoprenoids primarily suppress HMG-CoA reductase synthesis at a post-transcriptional level by attenuating HMG-CoA reductase mRNA translational efficiency. |
| Databáze: | OpenAIRE |
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