Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells
| Autor: | Shigeaki Ishizaka, Yukiteru Ouji, Akira Shiroi, Masahide Yoshikawa |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cellular differentiation
Biophysics Cell Communication Biology Biochemistry Inner root sheath Mice Proto-Oncogene Proteins Keratin medicine Animals Lymphocytes Molecular Biology Cells Cultured Cell Proliferation chemistry.chemical_classification Mice Inbred C3H integumentary system Wnt signaling pathway Cell Differentiation Epithelial Cells Cell Biology Hair follicle Keratin 1 Cell biology Wnt Proteins Keratin 5 medicine.anatomical_structure Epidermal Cells chemistry Immunology Loricrin Female Epidermis |
| Zdroj: | Biochemical and Biophysical Research Communications. 342:1063-1069 |
| ISSN: | 0006-291X |
| Popis: | Wnt-10b was originally isolated from lymphoid tissue and is known to be involved in a wide range of biological actions, while recently it was found to be expressed early in the development of hair follicles. However, few studies have been conducted concerning the role of Wnt-10b with the differentiation of skin epithelial cells. To evaluate its role in epithelial differentiation, we purified Wnt-10b from the supernatant of a concanavalin A-stimulated lymphocyte culture using an affinity column and investigated its effects on the differentiation of adult mouse-derived primary skin epithelial cells (MPSEC). MPSEC cultured with Wnt-10b showed morphological changes from cuboidal to spindle-shaped with inhibited proliferation, and also obtained characteristics of the hair shaft and inner root sheath of the hair follicle, represented by red-colored Ayoub Shklar staining, and reactions to AE-13 and AE-15 as seen with immunocytology. Further, RT-PCR analysis demonstrated the expression of mRNA for keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5, in Wnt-10b-treated MPSEC. In addition, involvement of the canonical Wnt signal pathway was demonstrated by a TCF reporter (pTOPFLASH) assay. These results suggest that Wnt-10b promotes the differentiation of MPSEC and may play an important role in hair follicle development by promoting differentiation of epithelial cells. |
| Databáze: | OpenAIRE |
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